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One of the two isolated mutants was damaged in the <jats:italic>COX5<\/jats:italic> gene, which encodes a subunit of the cytochrome <jats:italic>c<\/jats:italic> oxidase. BLAST searches in the genome of <jats:italic>Sc. stipitis<\/jats:italic> revealed that only one singular <jats:italic>COX5<\/jats:italic> gene exists in <jats:italic>Sc. stipitis<\/jats:italic>, in contrast to <jats:italic>Saccharomyces cerevisiae<\/jats:italic>, where two homologous genes are present. Mutant cells had lost the ability to grow with the amino acids glutamate, proline or aspartate and other non\u2010fermentable carbon sources, such as acetic acid and ethanol, as sole carbon sources. Biomass formation of the mutant cells in medium containing glucose or xylose as carbon source was lower compared with the wild\u2010type cells. However, yields and specific ethanol formation of the mutant were much higher, especially under conditions of higher aeration. The mutant cells lacked both cytochrome <jats:italic>c<\/jats:italic> oxidase activity and cyanide\u2010sensitive respiration, whereas ADH and PDC activities were distinctly enhanced. SHAM\u2010sensitive respiration was obviously essential for the fermentative metabolism, because SHAM completely abolished growth of the mutant cells with both glucose or xylose as carbon source. Copyright \u00a9 2011 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/yea.1840","type":"journal-article","created":{"date-parts":[[2010,12,9]],"date-time":"2010-12-09T07:08:25Z","timestamp":1291878505000},"page":"309-320","source":"Crossref","is-referenced-by-count":6,"title":["A mutation in the <i>COX5<\/i> gene of the yeast <i>Scheffersomyces stipitis<\/i> alters utilization of amino acids as carbon source, ethanol formation and activity of cyanide insensitive respiration"],"prefix":"10.1002","volume":"28","author":[{"given":"Stefan","family":"Freese","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Volkmar","family":"Passoth","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ulrich","family":"Klinner","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2011,1,13]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0968-0004(98)01298-5"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1006\/mben.2001.0181"},{"key":"e_1_2_5_4_1","volume-title":"Current Protocols in Molecular Biology","author":"Ausubel FM","year":"1991"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ibiod.2008.03.007"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02046758"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02046695"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.23.14705"},{"key":"e_1_2_5_9_1","doi-asserted-by":"crossref","first-page":"517","DOI":"10.1093\/genetics\/133.3.517","article-title":"Mutations in the MGI1 genes convert Kluyveromyces lactis into a petite\u2010positive yeast","volume":"133","author":"Chen XJ","year":"1993","journal-title":"Genetics"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.82.8.2235"},{"key":"e_1_2_5_11_1","first-page":"205","volume-title":"The Yeasts","author":"Gancedo C","year":"1989"},{"key":"e_1_2_5_12_1","unstructured":"HagedornJ.1990.Isolierung und Charakterisierung von Mutanten im Xylosestoffwechsel und Entwicklung eines Transformationssystems f\u00fcr die HefePichia stipitis. 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