{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T08:35:14Z","timestamp":1789979714385,"version":"4.0.1"},"reference-count":24,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2011,6,15]],"date-time":"2011-06-15T00:00:00Z","timestamp":1308096000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Yeast"],"published-print":{"date-parts":[[2011,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The ability of<jats:italic>Candida albicans<\/jats:italic>to form drug\u2010resistant biofilms is an important factor in its contribution to human disease. Assays to identify and characterize molecules with activity against fungal biofilms are crucial for the development of drugs with improved anti\u2010biofilm activity. Here we report the application of an adenylate kinase (AK)\u2010based cytotoxicity assay of fungal cell lysis to the characterization of agents active against<jats:italic>C. albicans<\/jats:italic>biofilms. We have developed three protocols for the AK assay. The first measures AK activity in the supernatants of biofilms treated with antifungal drugs and can be performed in parallel with a standard 2,3\u2010bis\u2010(2\u2010methoxy\u20104\u2010nitro\u20105\u2010sulphophenyl)\u20102<jats:italic>H<\/jats:italic>\u2010tetrazolium\u20105\u2010caboxanilide\u2010based biofilm susceptibility assay; a second, more sensitive protocol measures the AK activity present within the biofilm matrix; and a third procedure allows the direct visualization of lytic activity toward biofilms formed on catheter material. Amphotericin B and caspofungin, the two most effective anti\u2010biofilm drugs currently used to treat fungal infections, both directly lyse planktonic<jats:italic>C. albicans<\/jats:italic>cells<jats:italic>in vitro<\/jats:italic>, leading to the release of AK into the culture medium. These studies serve to validate the AK\u2010based lysis assay as a useful addition to the methods for the characterization of antifungal agents active toward biofilms and provide insights into the mode of action of amphotericin B and caspofungin against<jats:italic>C. albicans<\/jats:italic>biofilms. Copyright \u00a9 2011 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/yea.1860","type":"journal-article","created":{"date-parts":[[2011,5,17]],"date-time":"2011-05-17T02:25:45Z","timestamp":1305599145000},"page":"561-568","source":"Crossref","is-referenced-by-count":32,"title":["A novel assay of biofilm antifungal activity reveals that amphotericin B and caspofungin lyse<i>Candida albicans<\/i>cells in biofilms"],"prefix":"10.1002","volume":"28","author":[{"given":"Louis","family":"DiDone","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Duana","family":"Oga","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Damian J.","family":"Krysan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2011,6,15]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.183.18.5385-5394.2001"},{"key":"e_1_2_5_3_1","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1093\/oso\/9780195148091.003.0003","volume-title":"Clinical Mycology","author":"Chapman SW","year":"2003"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2010.47"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0966-842X(02)00002-1"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00316-09"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.62.3.915-921.1994"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid1001.030119"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-2008-1063859"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.17.2.255-267.2004"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1177\/1087057108320713"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.46.6.1773-1780.2002"},{"key":"e_1_2_5_13_1","first-page":"186","article-title":"Candida biofilms: antifungal resistance and emerging therapeutic options","volume":"5","author":"Kuhn DM","year":"2004","journal-title":"Curr Opin Investig Drugs"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.45.4.999-1007.2001"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02273-10"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1086\/651200"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1086\/596757"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2008.141"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1097\/QCO.0b013e32833e5850"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.3109\/10408410903241436"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.45.9.2475-2479.2001"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.46.11.3634-3636.2002"},{"key":"e_1_2_5_23_1","doi-asserted-by":"publisher","DOI":"10.1080\/13693780701581458"},{"key":"e_1_2_5_24_1","doi-asserted-by":"publisher","DOI":"10.1128\/EC.4.8.1493-1502.2005"},{"key":"e_1_2_5_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0507.2009.01690.x"}],"container-title":["Yeast"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fyea.1860","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/yea.1860","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,4,6]],"date-time":"2024-04-06T19:04:04Z","timestamp":1712430244000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/yea.1860"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,6,15]]},"references-count":24,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2011,8]]}},"alternative-id":["10.1002\/yea.1860"],"URL":"https:\/\/doi.org\/10.1002\/yea.1860","archive":["Portico"],"relation":{},"ISSN":["0749-503X","1097-0061"],"issn-type":[{"value":"0749-503X","type":"print"},{"value":"1097-0061","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011,6,15]]}}}