{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T19:24:35Z","timestamp":1791228275679,"version":"4.1.0"},"reference-count":22,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2011,12,2]],"date-time":"2011-12-02T00:00:00Z","timestamp":1322784000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Yeast"],"published-print":{"date-parts":[[2012,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Extraction of high molecular weight chromosomal DNA from yeast cells is a procedure that is performed frequently for experiments involving polymerase chain reaction (PCR), Southern blotting and other DNA analysis techniques. We have investigated several parameters affecting DNA yield and quality, using a simple chemical\u2010based purification procedure that was modelled on alkaline lysis methods developed for bacterial cells. The three major steps of the procedure, cell lysis, protein removal and DNA precipitation, were optimized by testing the impacts of several chemicals, including sodium dodecyl sulphate (SDS), sodium hydroxide, Tris buffer, sodium acetate and potassium acetate. Other parameters, such as the effect of elevated temperatures on cell lysis, were also investigated. A rapid, optimized protocol was derived for the purification of DNA from small cell cultures that can be readily digested with restriction enzymes and used as a template for PCR. Average yield was calculated to be approximately 1.7 \u00b5g DNA\/10\n                    <jats:sup>8<\/jats:sup>\n                    cells, which is similar to the theoretical maximum amount obtainable from haploid yeast cells. Copyright \u00a9 2011 John Wiley &amp; Sons, Ltd.\n                  <\/jats:p>","DOI":"10.1002\/yea.1918","type":"journal-article","created":{"date-parts":[[2011,11,15]],"date-time":"2011-11-15T22:46:27Z","timestamp":1321397187000},"page":"73-80","source":"Crossref","is-referenced-by-count":18,"title":["Factors affecting chemical\u2010based purification of DNA from\n                    <i>Saccharomyces cerevisiae<\/i>"],"prefix":"10.1002","volume":"29","author":[{"given":"Christopher K.","family":"Lee","sequence":"first","affiliation":[{"name":"Department of Chemistry and Biochemistry Texas State University  San Marcos USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naoko","family":"Araki","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biochemistry Texas State University  San Marcos USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Drew S.","family":"Sowersby","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biochemistry Texas State University  San Marcos USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L. Kevin","family":"Lewis","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biochemistry Texas State University  San Marcos USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2011,12,2]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.2144\/00284st02"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11046-010-9284-7"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0061(19980130)14:2<115::AID-YEA204>3.0.CO;2-2"},{"key":"e_1_2_6_5_1","volume-title":"Methods in Yeast Genetics","author":"Burke D","year":"2000"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0061(19970915)13:11<1065::AID-YEA159>3.0.CO;2-K"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1006\/abio.1993.1346"},{"key":"e_1_2_6_8_1","first-page":"538","article-title":"Simplified isolation of chromosomal and plasmid DNA from yeasts","volume":"14","author":"Fujimura H","year":"1993","journal-title":"Biotechniques"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.274.5287.546"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1186\/1472-6750-4-8"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(87)90131-4"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1389-1723(03)90133-2"},{"key":"e_1_2_6_13_1","first-page":"677","article-title":"Modified protocol for yeast DNA mini\u2010preparation","volume":"12","author":"Lee FJ","year":"1992","journal-title":"Biotechniques"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.18.4.1891"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.2144\/000113672"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(89)90360-6"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/16.1.364"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/19.19.5443"},{"key":"e_1_2_6_19_1","volume-title":"Molecular Cloning: A Laboratory Manual","author":"Sambrook J","year":"2001"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(02)50954-X"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00351857"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.E05-01-0053"},{"key":"e_1_2_6_23_1","doi-asserted-by":"crossref","first-page":"592","DOI":"10.2144\/97234bm06","article-title":"Improved alkaline lysis method for rapid isolation of plasmid DNA","volume":"23","author":"Zhou A","year":"1997","journal-title":"Biotechniques"}],"container-title":["Yeast"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fyea.1918","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/yea.1918","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,23]],"date-time":"2023-09-23T10:35:56Z","timestamp":1695465356000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/yea.1918"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,12,2]]},"references-count":22,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2012,2]]}},"alternative-id":["10.1002\/yea.1918"],"URL":"https:\/\/doi.org\/10.1002\/yea.1918","archive":["Portico"],"relation":{},"ISSN":["0749-503X","1097-0061"],"issn-type":[{"value":"0749-503X","type":"print"},{"value":"1097-0061","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011,12,2]]}}}