{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T01:45:24Z","timestamp":1785548724902,"version":"3.56.0"},"reference-count":27,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2012,10,14]],"date-time":"2012-10-14T00:00:00Z","timestamp":1350172800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Yeast"],"published-print":{"date-parts":[[2012,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>The limited number of treatments for oral candidiasis resulted in the emergence of azole\u2010resistant <jats:italic>Candida albicans<\/jats:italic> strains, thus enforcing the need for novel antifungal treatments. Although histatin 5 (H5) demonstrates antifungal activity, its inhibitory effect when adhered to hydroxyapatite and Polymetylmethacrylate (PMMA) surfaces, resembling conditions of the <jats:italic>in vivo<\/jats:italic> pellicle, remains unexplored. The objective of this <jats:italic>in vitro<\/jats:italic> study was to determine whether surface\u2010adhered H5 inhibits the colonization of <jats:italic>C. albicans<\/jats:italic> on hydroxyapatite and\/or PMMA. The <jats:italic>C. albicans<\/jats:italic> assay involved developing a mono\u2010protein pellicle (either H5 or albumin) on hydroxyapatite and PMMA discs, introducing <jats:italic>C. albicans<\/jats:italic> and counting the number of adhered cells, throughout time, using scanning electron microscopy. A negative binomial statistical model and the Tukey\u2013Kramer test were used for statistical analysis, with <jats:italic>p\u2009&lt;<\/jats:italic>\u20090.01 indicating significance. H5\u2010coated PMMA had significantly reduced number of cells compared to albumin\u2010coated PMMA at 30, 90 and 1440 min (<jats:italic>p\u2009&lt;<\/jats:italic>\u20090.0001), with the number of cells decreasing significantly in 90 and 1440 min (<jats:italic>p\u2009&lt;<\/jats:italic>\u20090.0001). Similarly, H5\u2010coated hydroxyapatite had significantly fewer cells compared to the albumin\u2010coated surface at 90 and 1440 min (<jats:italic>p\u2009&lt;<\/jats:italic>\u20090.0001), with the number of cells decreasing significantly at 30, 90 and 1440 min (<jats:italic>p\u2009&lt;<\/jats:italic>\u20090.0001). In conclusion, <jats:italic>C. albicans<\/jats:italic> colonization was most inhibited by PMMA and hydroxyapatite\u2010adhered H5 after 1440 min, illustrating the time\u2010dependent effect of H5. In addition, yeast cells colonized albumin\u2010coated PMMA, while dense hyphal networks formed on albumin\u2010coated hydroxyapatite, suggesting that <jats:italic>C. albicans<\/jats:italic> morphology is influenced by the surface available for albumin adhesion. Copyright \u00a9 2012 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/yea.2925","type":"journal-article","created":{"date-parts":[[2012,9,22]],"date-time":"2012-09-22T00:09:09Z","timestamp":1348272549000},"page":"459-466","source":"Crossref","is-referenced-by-count":23,"title":["The effect of histatin 5, adsorbed on PMMA and hydroxyapatite, on <i>Candida albicans<\/i> colonization"],"prefix":"10.1002","volume":"29","author":[{"given":"D.","family":"Vukosavljevic","sequence":"first","affiliation":[{"name":"Schulich School of Medicine and Dentistry University of Western Ontario  Dental Sciences Building DSB0071 London ON Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W.","family":"Custodio","sequence":"additional","affiliation":[{"name":"Schulich School of Medicine and Dentistry University of Western Ontario  Dental Sciences Building DSB0071 London ON Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. A.","family":"Del Bel Cury","sequence":"additional","affiliation":[{"name":"Piracicaba Dental School, State University of Campinas Department of Prosthodontics and Periodontology  Avenida Limeira 901 Piracicaba S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W. L.","family":"Siqueira","sequence":"additional","affiliation":[{"name":"Schulich School of Medicine and Dentistry University of Western Ontario  Dental Sciences Building DSB0071 London ON Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2012,10,14]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0811994106"},{"key":"e_1_2_6_3_1","first-page":"252","article-title":"Surface roughness of enamel and four resin composites","volume":"22","author":"Botta AC","year":"2009","journal-title":"Am J Dent"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2009.11.003"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1177\/00220345950740050301"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.183.18.5385-5394.2001"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0765.1990.tb01202.x"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.820291015"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2842.2007.01709.x"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0507.2010.01862.x"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00784-002-0176-1"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.archoralbio.2011.04.006"},{"key":"e_1_2_6_13_1","first-page":"175","article-title":"The antimicrobial peptide histatin 5 releases cellular materials from Candida albicans","volume":"79","author":"Gyurko C","year":"2000","journal-title":"J Dent Res"},{"key":"e_1_2_6_14_1","first-page":"29","article-title":"The structure, function and properties of the acquired pellicle","volume":"19","author":"Hannig M","year":"2006","journal-title":"Monogr Oral Sci"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-3913(05)80357-9"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10482-010-9417-5"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2005.00616.x"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1522-2683(19990801)20:11<2299::AID-ELPS2299>3.0.CO;2-7"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1439-0507.2000.00564.x"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chemgeo.2009.01.012"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.archoralbio.2009.01.016"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1567-1364.2006.00117.x"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1601-0825.2000.tb00106.x"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1021\/pr060580k"},{"key":"e_1_2_6_25_1","first-page":"67","article-title":"Candidacidal activity and helical conformation of active C\u2010terminal fragments of human salivary histatin\u20105","volume":"22","author":"Tsai H","year":"1997","journal-title":"Int J Oral Biol"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12079-011-0151-1"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkh181"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/415389a"}],"container-title":["Yeast"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fyea.2925","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/yea.2925","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,7]],"date-time":"2023-10-07T13:02:59Z","timestamp":1696683779000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/yea.2925"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,10,14]]},"references-count":27,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2012,11]]}},"alternative-id":["10.1002\/yea.2925"],"URL":"https:\/\/doi.org\/10.1002\/yea.2925","archive":["Portico"],"relation":{},"ISSN":["0749-503X","1097-0061"],"issn-type":[{"value":"0749-503X","type":"print"},{"value":"1097-0061","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,10,14]]}}}