{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,2]],"date-time":"2025-07-02T18:14:56Z","timestamp":1751480096027},"reference-count":39,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2013,1,30]],"date-time":"2013-01-30T00:00:00Z","timestamp":1359504000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Yeast"],"published-print":{"date-parts":[[2013,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Kl<\/jats:italic>Pdr1p is a single <jats:italic>Kluyveromyces lactis<\/jats:italic> homologue of <jats:italic>Saccharomyces cerevisiae Sc<\/jats:italic>Pdr1p\/<jats:italic>Sc<\/jats:italic>Pdr3p, the main transcriptional regulators of genes involved in <jats:italic>S. cerevisiae<\/jats:italic> multidrug resistance. <jats:italic>KlPDR1<\/jats:italic> deletion leads to a sharp increase in <jats:italic>K. lactis<\/jats:italic> drug susceptibility. The presence of putative PDRE and YRE regulatory elements in the <jats:italic>KlPDR1<\/jats:italic> gene promoter suggests an autoregulation of its transcription as well as its control by <jats:italic>Kl<\/jats:italic>Yap1p, the transcription factor involved in oxidative stress response. In this study, one plasmid\u2010borne <jats:italic>Klpdr1\u20101<\/jats:italic> allele that led to amino acid substitution (L273P) in the <jats:italic>Kl<\/jats:italic>Pdr1p was isolated. Overexpression of the <jats:italic>Klpdr1\u20101<\/jats:italic> allele from a multicopy plasmid in the <jats:italic>K. lactis<\/jats:italic> wild\u2010type and <jats:italic>Klpdr1\u0394<\/jats:italic> mutant strain increased the tolerance of transformants to oligomycin. The plasmid\u2010borne <jats:italic>Klpdr1\u20101<\/jats:italic> allele increased the activation of the <jats:italic>ScPDR5<\/jats:italic> promoter and complemented the drug hypersensitivity of the <jats:italic>S. cerevisiae pdr1\u0394 pdr3\u0394<\/jats:italic> mutant strain. The results indicate that L273P amino acid substitution is the result of a gain\u2010of\u2010function mutation in the <jats:italic>KlPDR1<\/jats:italic> gene that confers <jats:italic>Kl<\/jats:italic>Pdr1p hyperactivity, as revealed by a high expression of the ABC transporter gene <jats:italic>KlPDR5<\/jats:italic>, leading to multidrug resistance and rhodamine 6G efflux out of the cells. Copyright \u00a9 2013 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/yea.2941","type":"journal-article","created":{"date-parts":[[2013,1,9]],"date-time":"2013-01-09T18:59:36Z","timestamp":1357757976000},"page":"71-80","source":"Crossref","is-referenced-by-count":1,"title":["Gain\u2010of\u2010function mutation in the <i>KlPDR1<\/i> gene encoding multidrug resistance regulator in <i>Kluyveromyces lactis<\/i>"],"prefix":"10.1002","volume":"30","author":[{"given":"Zuzana","family":"Balazfyova","sequence":"first","affiliation":[{"name":"Department of Microbiology and Virology Comenius University in Bratislava  Slovak Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nora Toth","family":"Hervay","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Virology Comenius University in Bratislava  Slovak 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