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This gene is also involved in the regulation of multidrug resistance in <jats:italic>Saccharomyces cerevisiae<\/jats:italic> and pathogenic yeasts. Here we report the results of functional analysis of the <jats:italic>CgPDR16<\/jats:italic> gene, whose mutation has been previously shown to enhance fluconazole sensitivity in <jats:italic>Candida glabrata<\/jats:italic> mutant cells. We have cloned the <jats:italic>CgPDR16<\/jats:italic> gene, which was able to complement the <jats:italic>pdr16\u0394<\/jats:italic> mutation in both <jats:italic>C. glabrata<\/jats:italic> and <jats:italic>S. cerevisiae<\/jats:italic>. Along with fluconazole, the <jats:italic>pdr16\u0394<\/jats:italic> mutation resulted in increased susceptibility of mutant cells to several azole antifungals without changes in sensitivity to polyene antibiotics, cycloheximide, NQO, 5\u2010fluorocytosine and oxidants inducing the intracellular formation of reactive oxygen species. The susceptibility of the <jats:italic>pdr16\u0394<\/jats:italic> mutant strain to itraconazole and 5\u2010fluorocytosine was enhanced by CTBT [7\u2010chlorotetrazolo(5,1\u2010c)benzo(1,2,4)triazine] inducing oxidative stress. The <jats:italic>pdr16\u0394<\/jats:italic> mutation increased the accumulation of rhodamine 6G in mutant cells, decreased the level of itraconazole resistance caused by gain\u2010of\u2010function mutations in the <jats:italic>CgPDR1<\/jats:italic> gene, and reduced cell surface hydrophobicity and biofilm production. These results point to the pleiotropic phenotype of the <jats:italic>pdr16\u0394<\/jats:italic> mutant and support the role of the <jats:italic>CgPDR16<\/jats:italic> gene in the control of drug susceptibility and virulence in the pathogenic <jats:italic>C. glabrata<\/jats:italic>. Copyright \u00a9 2013 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/yea.2978","type":"journal-article","created":{"date-parts":[[2013,8,12]],"date-time":"2013-08-12T12:57:47Z","timestamp":1376312267000},"page":"403-414","source":"Crossref","is-referenced-by-count":19,"title":["Mutation of the <i>CgPDR16<\/i> gene attenuates azole tolerance and biofilm production in pathogenic <i>Candida glabrata<\/i>"],"prefix":"10.1002","volume":"30","author":[{"given":"Hana","family":"Culakova","sequence":"first","affiliation":[{"name":"Department of Microbiology and Virology, Faculty of Natural Sciences Comenius University in Bratislava  842 15 Bratislava Slovak Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vladimira","family":"Dzugasova","sequence":"additional","affiliation":[{"name":"Department of Genetics, Faculty of Natural Sciences Comenius University in Bratislava  842 15 Bratislava Slovak 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