{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,4]],"date-time":"2025-12-04T18:35:29Z","timestamp":1764873329005,"version":"3.37.3"},"reference-count":33,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2014,7,11]],"date-time":"2014-07-11T00:00:00Z","timestamp":1405036800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001870","name":"Foundation For Polish Science","doi-asserted-by":"publisher","award":["POMOST\/2010-2\/4"],"award-info":[{"award-number":["POMOST\/2010-2\/4"]}],"id":[{"id":"10.13039\/501100001870","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Yeast"],"published-print":{"date-parts":[[2014,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A BLAST search of the <jats:italic>Candida<\/jats:italic> Genome Database with the <jats:italic>Saccharomyces cerevisiae LYS4<\/jats:italic> sequence known to encode homoaconitase (HA) revealed ORFs 19.3846 and 19.11327. Both alleles of the <jats:italic>LYS4<\/jats:italic> gene were sequentially disrupted in <jats:italic>Candida albicans<\/jats:italic> BWP17 cells using PCR\u2010based methodology. The null <jats:italic>lys4<\/jats:italic>\u0394 mutant exhibited lysine auxotrophy in minimal medium but was able to grow in the presence of <jats:sc>l<\/jats:sc>\u2010Lys and <jats:italic>\u03b1<\/jats:italic>\u2010aminoadipate, an intermediate of the <jats:italic>\u03b1<\/jats:italic>\u2010aminoadipate pathway, at millimolar concentrations. The presence of <jats:sc>d<\/jats:sc>\u2010Lys and pipecolic acid did not trigger <jats:italic>lys4<\/jats:italic>\u0394 growth. The <jats:italic>C<\/jats:italic>. <jats:italic>albicans lys4<\/jats:italic>\u0394 mutant cells demonstrated diminished germination ability. However, their virulence <jats:italic>in vivo<\/jats:italic> in a murine model of disseminated neonatal candidiasis appeared identical to that of the wild\u2010type strain. Moreover, there was no statistically significant difference in fungal burden of infected tissues between the strains. Copyright \u00a9 2014 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/yea.3021","type":"journal-article","created":{"date-parts":[[2014,6,5]],"date-time":"2014-06-05T04:24:51Z","timestamp":1401942291000},"page":"299-308","source":"Crossref","is-referenced-by-count":5,"title":["Phenotypic consequences of <i>LYS4<\/i> gene disruption in <i>Candida albicans<\/i>"],"prefix":"10.1002","volume":"31","author":[{"given":"Iwona","family":"Gabriel","sequence":"first","affiliation":[{"name":"Department of Pharmaceutical Technology and Biochemistry Gda\u0144sk University of Technology  Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Krzysztof","family":"Kur","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Technology and Biochemistry Gda\u0144sk University of Technology  Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sonia S.","family":"Laforce\u2010Nesbitt","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Women and Infants Hospital of Rhode Island Brown University  Providence RI USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anoop S.","family":"Pulickal","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Women and Infants Hospital of Rhode Island Brown University  Providence RI USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7425-1895","authenticated-orcid":false,"given":"Joseph M.","family":"Bliss","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Women and Infants Hospital of Rhode Island Brown University  Providence RI USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S\u0142awomir","family":"Milewski","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Technology and Biochemistry Gda\u0144sk University of Technology  Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2014,7,11]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-280X.1999.00246.x"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9861(68)90129-X"},{"volume-title":"Reference Method for Broth Dilution Antifungal Susceptibility Testing of Yeasts; 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