{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,18]],"date-time":"2025-10-18T15:02:19Z","timestamp":1760799739432,"version":"3.37.3"},"reference-count":37,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2014,9,15]],"date-time":"2014-09-15T00:00:00Z","timestamp":1410739200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100002911","name":"Universidad Complutense de Madrid","doi-asserted-by":"publisher","award":["G35\/10-A"],"award-info":[{"award-number":["G35\/10-A"]}],"id":[{"id":"10.13039\/501100002911","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003176","name":"Ministerio de Educaci\u00f3n, Cultura y Deporte","doi-asserted-by":"publisher","award":["FPU Predoctoral fellowship"],"award-info":[{"award-number":["FPU Predoctoral fellowship"]}],"id":[{"id":"10.13039\/501100003176","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002911","name":"Universidad Complutense de Madrid","doi-asserted-by":"publisher","award":["PICATA predoctoral fellowship"],"award-info":[{"award-number":["PICATA predoctoral fellowship"]}],"id":[{"id":"10.13039\/501100002911","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Yeast"],"published-print":{"date-parts":[[2014,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The colony shape of four yeast species growing on agar medium was measured for 116\u2009days by image analysis. Initially, all the colonies are circular, with regular edges. The loss of circularity can be quantitatively estimated by the eccentricity index, <jats:italic>E<\/jats:italic><jats:sub>i<\/jats:sub>, calculated as the ratio between their orthogonal vertical and horizontal diameters. <jats:italic>E<\/jats:italic><jats:sub>i<\/jats:sub> can increase from 1 (complete circularity) to a maximum of 1.17\u20131.30, depending on the species. One colony inhibits its neighbour only when it has reached a threshold area. Then, <jats:italic>E<\/jats:italic><jats:sub>i<\/jats:sub> of the inhibited colony increases proportionally to the area of the inhibitory colony. The initial distance between colonies affects those threshold values but not the proportionality, <jats:italic>E<\/jats:italic><jats:sub>i<\/jats:sub>\/area; this inhibition affects the shape but not the total surface of the colony. The appearance of irregularities in the edges is associated, in all the species, not with age but with nutrient exhaustion. The edge irregularity can be quantified by the Fourier index, <jats:italic>F<\/jats:italic><jats:sub>i<\/jats:sub>, calculated by the minimum number of Fourier coefficients that are needed to describe the colony contour with 99% fitness. An <jats:italic>ad hoc<\/jats:italic> function has been developed in Matlab v. 7.0 to automate the computation of the Fourier coefficients. In young colonies, <jats:italic>F<\/jats:italic><jats:sub>i<\/jats:sub> has a value between 2 (circumference) and 3 (ellipse). These values are maintained in mature colonies of <jats:italic>Debaryomyces<\/jats:italic>, but can reach values up to 14 in <jats:italic>Saccharomyces<\/jats:italic>. All the species studied showed the inhibition of growth in facing colony edges, but only three species showed edge irregularities associated with substrate exhaustion. Copyright \u00a9 2014 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/yea.3036","type":"journal-article","created":{"date-parts":[[2014,8,5]],"date-time":"2014-08-05T18:47:11Z","timestamp":1407264431000},"page":"431-440","source":"Crossref","is-referenced-by-count":8,"title":["Quantitative analysis of morphological changes in yeast colonies growing on solid medium: the eccentricity and Fourier indices"],"prefix":"10.1002","volume":"31","author":[{"given":"Elena","family":"Gil de Prado","sequence":"first","affiliation":[{"name":"Departamento de Microbiolog\u00eda III, Facultad de Biolog\u00eda Universidad Complutense de Madrid  Spain"},{"name":"CEI Campus Moncloa UCM\u2013UPM  Madrid Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eva\u2010Mar\u00eda","family":"Rivas","sequence":"additional","affiliation":[{"name":"Departamento de Microbiolog\u00eda III, Facultad de Biolog\u00eda Universidad Complutense de Madrid  Spain"},{"name":"CEI Campus Moncloa UCM\u2013UPM  Madrid Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mar\u00eda\u2010Isabel","family":"de Sil\u00f3niz","sequence":"additional","affiliation":[{"name":"Departamento de Microbiolog\u00eda III, Facultad de Biolog\u00eda Universidad Complutense de Madrid  Spain"},{"name":"CEI Campus Moncloa UCM\u2013UPM  Madrid Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bel\u00e9n","family":"Diezma","sequence":"additional","affiliation":[{"name":"LPF\u2013TAGRALIA, Departamento de Ingenier\u00eda Rural, ETSI Agr\u00f3nomos Universidad Polit\u00e9cnica de Madrid  Spain"},{"name":"CEI Campus Moncloa UCM\u2013UPM  Madrid Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pilar","family":"Barreiro","sequence":"additional","affiliation":[{"name":"LPF\u2013TAGRALIA, Departamento de Ingenier\u00eda Rural, ETSI Agr\u00f3nomos Universidad Polit\u00e9cnica de Madrid  Spain"},{"name":"CEI Campus Moncloa UCM\u2013UPM  Madrid Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jos\u00e9 M.","family":"Peinado","sequence":"additional","affiliation":[{"name":"Departamento de Microbiolog\u00eda III, Facultad de Biolog\u00eda Universidad Complutense de Madrid  Spain"},{"name":"CEI Campus Moncloa UCM\u2013UPM  Madrid Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2014,9,15]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fishres.2011.06.003"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0811816106"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/abio.370180103"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2006.07.028"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.compag.2005.09.004"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1086\/BBLv220n3p224"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mimet.2006.12.020"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0011008"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-7012(00)00142-1"},{"volume-title":"Digital Image Processing Using Matlab","year":"2004","author":"Gonzalez RC","key":"e_1_2_6_11_1"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1000823"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(04)00118-7"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-7012(02)00160-4"},{"volume-title":"Yeasts and Yeast\u2010like Organisms","year":"1990","author":"Kockov\u00e1\u2010Kratochvilov\u00e1 A","key":"e_1_2_6_15_1"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/0146-664X(82)90034-X"},{"volume-title":"The Yeasts. A Taxonomic Study","year":"2011","author":"Kurtzman CP","key":"e_1_2_6_17_1"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1128\/jb.178.24.7144-7151.1996"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.compag.2011.10.016"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-8-117"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fm.2012.04.001"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1109\/TSMC.1979.4310076"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/37398"},{"key":"e_1_2_6_24_1","doi-asserted-by":"crossref","first-page":"1923","DOI":"10.1242\/jcs.113.11.1923","article-title":"Yeast colonies synchronise their growth and development","volume":"113","author":"Palkov\u00e1 Z","year":"2000","journal-title":"J Cell Sci"},{"key":"e_1_2_6_25_1","first-page":"73","article-title":"Use of image analysis to map bacterial growth on solid media","volume":"2","author":"Peters AC","year":"1990","journal-title":"Binary Comput Microbiol"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1134\/S0026261710020025"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.291.5505.878"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1008816702162"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.femsyr.2004.09.002"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fgb.2010.08.005"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1155\/2012\/601836"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201103129"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1094\/ASBCJ-59-0069"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2006.05072.x"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.resmic.2005.05.012"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-2920.2009.02067.x"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijfoodmicro.2010.06.007"},{"key":"e_1_2_6_38_1","doi-asserted-by":"publisher","DOI":"10.1002\/bit.1164"}],"container-title":["Yeast"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fyea.3036","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/yea.3036","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,5]],"date-time":"2023-10-05T01:27:26Z","timestamp":1696469246000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/yea.3036"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,9,15]]},"references-count":37,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2014,11]]}},"alternative-id":["10.1002\/yea.3036"],"URL":"https:\/\/doi.org\/10.1002\/yea.3036","archive":["Portico"],"relation":{},"ISSN":["0749-503X","1097-0061"],"issn-type":[{"type":"print","value":"0749-503X"},{"type":"electronic","value":"1097-0061"}],"subject":[],"published":{"date-parts":[[2014,9,15]]}}}