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We show that puromycin\u2010resistance can be achieved in yeast through expression of a bacterial puromycin\u2010resistance gene optimized to the yeast codon bias, which in turn serves as an easy\u2010to\u2010use dominant genetic marker suitable for gene disruption. We have constructed a similar DNA cassette expressing yeast codon\u2010optimized mutant human dihydrofolate reductase (<jats:italic>DHFR<\/jats:italic>), which confers resistance to methotrexate and can also be used as a dominant selectable marker. Both of these drug\u2010resistant marker cassettes are flanked by <jats:italic>loxP<\/jats:italic> sites, allowing for their excision from the genome following expression of Cre\u2010recombinase. Finally, we have created a series of plasmids for low\u2010level constitutive expression of Cre\u2010recombinase in yeast that allows for efficient excision of <jats:italic>loxP<\/jats:italic>\u2010flanked markers. Copyright \u00a9 2015 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/yea.3069","type":"journal-article","created":{"date-parts":[[2015,6,4]],"date-time":"2015-06-04T18:33:18Z","timestamp":1433442798000},"page":"423-438","source":"Crossref","is-referenced-by-count":18,"title":["Puromycin\u2010 and methotrexate\u2010resistance cassettes and optimized Cre\u2010recombinase expression plasmids for use in yeast"],"prefix":"10.1002","volume":"32","author":[{"given":"Chris","family":"MacDonald","sequence":"first","affiliation":[{"name":"Department of Molecular Physiology and Biophysics University of Iowa  IA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert C.","family":"Piper","sequence":"additional","affiliation":[{"name":"Department of Molecular Physiology and Biophysics University of Iowa  IA 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