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Transformants containing a very high copy number of a P28\u2010I expression vector were selected by genetic complementation involving deficient <jats:italic>LEU2<\/jats:italic> or <jats:italic>URA3<\/jats:italic> alleles carried by plasmids. Using the <jats:italic>ura3<\/jats:italic> <jats:italic>fur1<\/jats:italic> auto\u2010selection system, constitutive and stable expression of P28\u2010I could be obtained in cultures grown in rich medium.<\/jats:p><jats:p>The accumulation of the foreign protein exceeds 25% of total yeast proteins when estimated by Coomassie Brilliant Blue staining of SDS\u2010PAGE. Moreover, P28\u2010I which was located intracellularly was soluble and biologically active.<\/jats:p>","DOI":"10.1002\/yea.320050609","type":"journal-article","created":{"date-parts":[[2005,5,28]],"date-time":"2005-05-28T23:25:24Z","timestamp":1117322724000},"page":"497-507","source":"Crossref","is-referenced-by-count":61,"title":["High level of expression of a protective antigen of schistosomes in <i>Saccharomyces cerevisiae<\/i>"],"prefix":"10.1002","volume":"5","author":[{"given":"G.","family":"Loison","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Vidal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Findeli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Roitsch","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. 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