{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,31]],"date-time":"2023-10-31T14:46:47Z","timestamp":1698763607006},"reference-count":45,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2004,1,29]],"date-time":"2004-01-29T00:00:00Z","timestamp":1075334400000},"content-version":"vor","delay-in-days":3621,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Yeast"],"published-print":{"date-parts":[[1994,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The location and sequence of the <jats:italic>PDA1<\/jats:italic> gene, encoding the E1\u03b1 subunit of the pyruvate dehydrogenase (PDH) complex from <jats:italic>Saccharomyces cerevisiae<\/jats:italic>, were determined. The <jats:italic>PDA1<\/jats:italic> gene was located on a 6\u00b72 kb fragment of chromosome V, approximately 18 kb centromere distal to <jats:italic>RAD3<\/jats:italic>. Consistent with this, the <jats:italic>PDA1<\/jats:italic> gene was genetically mapped at 4 cM from <jats:italic>RAD3<\/jats:italic>. A part of the 6\u00b72 kb fragment of chromosome V was sequenced. The nucleotide sequence contained the <jats:italic>PDA1<\/jats:italic> open reading frame and the entire putative promoter. Computer analysis revealed a putative GCN4 binding motif in the <jats:italic>PDA1<\/jats:italic> promoter. The presence of transcriptional elements was experimentally determined by deletion analysis. To this end, ExoIII deletions were constructed in the 5\u2032 to 3\u2032 direction of the <jats:italic>PDA1<\/jats:italic> promoter and effects on transcription were determined by Northern analysis. Transcription was unaffected upon deletion to position \u2212 190 relative to the ATG start codon. Deletions from position \u2212 148 and beyond, however, reduced promoter activity at least 40\u2010fold. Apparently the 42 bp between nucleotides \u2212 190 and \u2212 148 contain an element essential for transcription. Inactivation of the <jats:italic>PDA1<\/jats:italic> promoter could not be attributed to deletions of a recognizable TATA element or any known yeast regulatory motifs. The possible role of the CCCTT sequence present in the 42 bp region and also in the promoters of the other genes encoding subunits of the PDH complex is discussed.<\/jats:p>","DOI":"10.1002\/yea.320100303","type":"journal-article","created":{"date-parts":[[2005,5,28]],"date-time":"2005-05-28T23:47:37Z","timestamp":1117324057000},"page":"297-308","source":"Crossref","is-referenced-by-count":7,"title":["Promoter analysis of the <i>PDA1<\/i> gene encoding the E1\u03b1 subunit of the pyruvate dehydrogenase complex from <i>Saccharomyces cerevisiae<\/i>"],"prefix":"10.1002","volume":"10","author":[{"given":"Thibaut J.","family":"Wenzel","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anne\u2010Marie","family":"Zuurmond","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anneke","family":"Bergmans","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Johan A.","family":"Van Den Berg","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H.","family":"Yde Steensma","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,1,29]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0076-6879(91)94015-5"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.86.22.8732"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(89)91549-0"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(92)90446-J"},{"key":"e_1_2_1_6_1","doi-asserted-by":"crossref","first-page":"296","DOI":"10.1007\/BF00279803","article-title":"Positive regulation of the LPD1 gene of Saccharomyces cerevisiae by the HAP2\/HAP3\/HAP4 activation system","volume":"231","author":"Bowman S. 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