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When measured in the sub\u2010second time scale, however, there was no significant inhibition of glucose uptake, by cyanide, up to 200\u2010ms, clearly demonstrating that, in cyanide treated cells, glucose uptake was not linear for the first 5\u2010s.<\/jats:p><jats:p>After a 200\u2010ms exposure of untreated cells to radio\u2010labelled glucose, less than 10% of the intracellular label resided in soluble uncharged compounds. In cyanide\u2010treated cells up to 43% of the labelled compounds were uncharged, with a concurrent reduction of intracellular label residing in anionic compounds. The results suggest that, in the presence of 10 mM cyanide when respiration is inhibited, a reduction in the cellular ATP concentration causes a reduction in hexose\u2010kinase activity which results in an accumulation of internal free glucose, which in turn causes a reduction in net glucose transport.<\/jats:p>","DOI":"10.1002\/yea.320101204","type":"journal-article","created":{"date-parts":[[2005,5,28]],"date-time":"2005-05-28T23:48:23Z","timestamp":1117324103000},"page":"1553-1558","source":"Crossref","is-referenced-by-count":17,"title":["Respiratory inhibitors affect incorporation of glucose into <i>Saccharomyces cerevisiae<\/i> cells, but not the activity of glucose transport"],"prefix":"10.1002","volume":"10","author":[{"given":"Michael C.","family":"Walsh","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hans\u2010Peter","family":"Smits","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karel","family":"van Dam","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,1,29]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2736(72)90205-2"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.80.6.1730"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1128\/jb.175.11.3289-3294.1993"},{"key":"e_1_2_1_5_1","doi-asserted-by":"crossref","first-page":"567","DOI":"10.1042\/bj2870567","volume":"287","author":"Hogget J. 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