{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T19:02:03Z","timestamp":1791486123701,"version":"4.3.4"},"reference-count":53,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2004,1,29]],"date-time":"2004-01-29T00:00:00Z","timestamp":1075334400000},"content-version":"vor","delay-in-days":3103,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Yeast"],"published-print":{"date-parts":[[1995,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>We have cloned a new gene, <jats:italic>SCP160<\/jats:italic>, from <jats:italic>Saccharomyces cerevisiae<\/jats:italic>, the deduced amino acid sequence of which does not exhibit overall similarity to any known yeast protein. A weak resemblance between the C\u2010terminal part of the Scp160 protein and regulatory subunits of cAMP\u2010dependent protein kinases from eukaryotes as well as the pstB protein of <jats:italic>Escherichia coli<\/jats:italic> was observed. The <jats:italic>SCP160<\/jats:italic> gene resides on the left arm of chromosome X and codes for a polypeptide of molecular weight around 160 kDa. By immunofluorescence microscopy the Scp160 protein appears to be localized to the nuclear envelope and to the endoplasmic reticulum (ER). However, no signal sequence or membrane\u2010spanning region exists, suggesting that the Scp160 protein is attached to the cytoplasmic surface of the ER\u2013nuclear envelope membranes. Disruption of the <jats:italic>SCP160<\/jats:italic> gene is not lethal but results in cells of decreased viability, abnormal morphology and increased DNA content. This phenotype is not reversible by transformation with a plasmid carrying the wild\u2010type gene. Crosses of <jats:italic>SCP160<\/jats:italic> deletion mutant strains among each other or with unrelated strains lead to irregular segregation of genetic markers. Taken together the data suggest that the Scp160 protein is required during cell division for faithful partitioning of the ER\u2013nuclear envelope membranes which in <jats:italic>S. cerevisiae<\/jats:italic> enclose the duplicated chromosomes.<\/jats:p>","DOI":"10.1002\/yea.320111004","type":"journal-article","created":{"date-parts":[[2005,5,27]],"date-time":"2005-05-27T23:18:17Z","timestamp":1117235897000},"page":"929-944","source":"Crossref","is-referenced-by-count":57,"title":["Scp160p, a new yeast protein associated with the nuclear membrane and the endoplasmic reticulum, is necessary for maintenance of exact ploidy"],"prefix":"10.1002","volume":"11","author":[{"given":"Ulrike","family":"Wintersberger","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian","family":"K\u00fchne","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anneliese","family":"Karwan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2004,1,29]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.7.3.998"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2836(85)90377-8"},{"key":"e_1_2_1_4_1","first-page":"59","volume-title":"The Molecular Biology of the Yeast Saccharomyces: Life Cycle and Inheritance","author":"Byers B.","year":"1981"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.7.8.2653"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)82362-6"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/14.22.9213"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1993.tb06092.x"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/206093a0"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.91.17.8258"},{"key":"e_1_2_1_11_1","unstructured":"Haug T.andSkogen O. W.(1991). Mapping and function analysis of theSCP160gene ofSaccharomyces cerevisiae. Master thesis. University of Trondheim and University of Vienna."},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/0076-6879(87)55014-5"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1128\/jb.153.1.163-168.1983"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00293a008"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.98.3.922"},{"key":"e_1_2_1_16_1","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1093\/genetics\/124.1.57","article-title":"Pedigree analyses of yeast cells recovering from DNA damage allow assignment of lethal events to individual post\u2010treatment generations","volume":"124","author":"Klein F.","year":"1990","journal-title":"Genetics"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00290678"},{"key":"e_1_2_1_18_1","unstructured":"K\u00fchne C.(1988). Isolation und Charakterisierung der Gene f\u00fcr eine zellul\u00e4re reverse Transcriptase und eine Ribnonuclease H aus der B\u00e4ckerhefe. Doctoral dissertation. University of Agricultural Sciences. 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