{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,25]],"date-time":"2025-10-25T18:44:46Z","timestamp":1761417886011,"version":"3.37.3"},"reference-count":46,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2017,11,29]],"date-time":"2017-11-29T00:00:00Z","timestamp":1511913600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["KAKENHI \/ JP26450111"],"award-info":[{"award-number":["KAKENHI \/ JP26450111"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Yeast"],"published-print":{"date-parts":[[2018,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>In eukaryotes, the glycosylphosphatidylinositol (GPI) modification of many glycoproteins on the cell surface is highly conserved. The lipid moieties of GPI\u2010anchored proteins undergo remodelling processes during their maturation. To date, the products of the <jats:italic>PER1<\/jats:italic>, <jats:italic>GUP1<\/jats:italic> and <jats:italic>CWH43<\/jats:italic> genes of the yeast <jats:styled-content style=\"fixed-case\"><jats:italic>Saccharomyces cerevisiae<\/jats:italic><\/jats:styled-content> have been shown to be involved in the lipid remodelling. Here, we focus on the putative GPI remodelling pathway in the methylotrophic yeast <jats:italic>Ogataea minuta<\/jats:italic>. We found that the <jats:styled-content style=\"fixed-case\"><jats:italic>O. minuta<\/jats:italic><\/jats:styled-content> homologues of <jats:italic>PER1<\/jats:italic>, <jats:italic>GUP1<\/jats:italic> and <jats:italic>CWH43<\/jats:italic> are functionally compatible with those of <jats:styled-content style=\"fixed-case\"><jats:italic>S. cerevisiae<\/jats:italic><\/jats:styled-content>. Disruption of <jats:italic>GUP1<\/jats:italic> or <jats:italic>CWH43<\/jats:italic> in <jats:styled-content style=\"fixed-case\"><jats:italic>O. minuta<\/jats:italic><\/jats:styled-content> caused a growth defect under non\u2010permissive conditions. The <jats:italic><jats:styled-content style=\"fixed-case\">O. minuta<\/jats:styled-content> per1<\/jats:italic>\u0394 mutant exhibited a more fragile phenotype than the <jats:italic>gup1<\/jats:italic>\u0394 or <jats:italic>cwh43<\/jats:italic>\u0394 mutants. To address the role of GPI modification in <jats:styled-content style=\"fixed-case\"><jats:italic>O. minuta<\/jats:italic><\/jats:styled-content>, we assessed the effect of these mutations on the processing and localization of the <jats:styled-content style=\"fixed-case\"><jats:italic>O. minuta<\/jats:italic><\/jats:styled-content> homologues of the Gas1 protein; in <jats:styled-content style=\"fixed-case\"><jats:italic>S. cerevisiae<\/jats:italic><\/jats:styled-content>, Gas1p is an abundant and well\u2010characterized GPI\u2010anchored protein. We found that <jats:styled-content style=\"fixed-case\"><jats:italic>O. minuta<\/jats:italic><\/jats:styled-content> possesses two copies of the <jats:italic>GAS1<\/jats:italic> gene, which we designate <jats:italic>GAS1A<\/jats:italic> and <jats:italic>GAS1B<\/jats:italic>. Microscopy and western blotting analysis showed mislocalization and\/or lower retention of Gas1Ap and Gas1Bp within the membrane fraction in <jats:italic>per1<\/jats:italic>\u0394 or <jats:italic>gup1<\/jats:italic>\u0394 mutant cells, suggesting the significance of lipid remodelling for GPI\u2010anchored proteins in <jats:styled-content style=\"fixed-case\"><jats:italic>O. minuta<\/jats:italic><\/jats:styled-content>. Localization behaviour of Gas1Bp differed from that of Gas1Ap. Our data reveals, for the first time (to our knowledge), the existence of genes related to GPI anchor remodelling in <jats:styled-content style=\"fixed-case\"><jats:italic>O. minuta<\/jats:italic><\/jats:styled-content> cells.<\/jats:p>","DOI":"10.1002\/yea.3285","type":"journal-article","created":{"date-parts":[[2017,10,13]],"date-time":"2017-10-13T09:56:34Z","timestamp":1507888594000},"page":"225-236","source":"Crossref","is-referenced-by-count":5,"title":["<i>PER1<\/i>, <i>GUP1<\/i> and <i>CWH43<\/i> of methylotrophic yeast <i>Ogataea minuta<\/i> are involved in cell wall integrity"],"prefix":"10.1002","volume":"35","author":[{"given":"Xin\u2010Xin","family":"Xu","sequence":"first","affiliation":[{"name":"Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education Jiangnan University  Wuxi 214122 China"},{"name":"Biotechnology Research Institute for Drug Discovery National Institute of Advanced Industrial Science and Technology (AIST)  Umezono Tsukuba 305\u20108568 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Akiko","family":"Komatsuzaki","sequence":"additional","affiliation":[{"name":"Biotechnology Research Institute for Drug Discovery National Institute of Advanced Industrial Science and Technology (AIST)  Umezono Tsukuba 305\u20108568 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yasunori","family":"Chiba","sequence":"additional","affiliation":[{"name":"Biotechnology Research Institute for Drug Discovery National Institute of Advanced Industrial Science and Technology (AIST)  Umezono Tsukuba 305\u20108568 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8575-5095","authenticated-orcid":false,"given":"Xiao\u2010Dong","family":"Gao","sequence":"additional","affiliation":[{"name":"Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education Jiangnan University  Wuxi 214122 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0291-8299","authenticated-orcid":false,"given":"Takehiko","family":"Yoko\u2010o","sequence":"additional","affiliation":[{"name":"Biotechnology Research Institute for Drug Discovery National Institute of Advanced Industrial Science and Technology (AIST)  Umezono Tsukuba 305\u20108568 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,11,29]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.00463-07"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/glycob\/cwp080"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.E06-02-0104"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(92)90189-J"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0061(199712)13:15<1477::AID-YEA184>3.0.CO;2-L"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.E11-04-0294"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0854.2008.00857.x"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.282.5389.699"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1992.tb05075.x"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrg2811"},{"key":"e_1_2_7_12_1","doi-asserted-by":"crossref","first-page":"26365","DOI":"10.1016\/S0021-9258(19)74324-5","article-title":"Structures of glycosylphosphatidylinositol membrane anchors from Saccharomyces cerevisiae","volume":"268","author":"Fankhauser C.","year":"1993","journal-title":"The Journal of Biological Chemistry"},{"key":"e_1_2_7_13_1","doi-asserted-by":"crossref","first-page":"2799","DOI":"10.1242\/jcs.112.17.2799","article-title":"The structure, biosynthesis and functions of glycosylphosphatidylinositol anchors, and the contributions of trypanosome research","volume":"112","author":"Ferguson M. 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