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Over one third of the discovered genes are not present in the <jats:styled-content style=\"fixed-case\"><jats:italic>S.\u00a0cerevisiae<\/jats:italic><\/jats:styled-content> core genome but instead are often restricted to a subset of yeast isolates and thus may be important for adaptation to specific environmental niches. We refer to these genes as \u201cpan\u2010genes,\u201d being part of the pangenome but not the core genome.<\/jats:p><jats:p>Here, we describe the evolutionary journey and characterisation of a novel pan\u2010gene, originally named hypothetical (<jats:italic>HYPO<\/jats:italic>) open\u2010reading frame. Phylogenetic analysis reveals that <jats:italic>HYPO<\/jats:italic> has been predominantly retained in <jats:styled-content style=\"fixed-case\"><jats:italic>S.\u00a0cerevisiae<\/jats:italic><\/jats:styled-content> strains associated with brewing but has been repeatedly lost in most other fungal species during evolution. There is also evidence that <jats:italic>HYPO<\/jats:italic> was horizontally transferred at least once, from <jats:styled-content style=\"fixed-case\"><jats:italic>S.\u00a0cerevisiae<\/jats:italic><\/jats:styled-content> to <jats:styled-content style=\"fixed-case\"><jats:italic>Saccharomyces paradoxus<\/jats:italic><\/jats:styled-content>. The phylogenetic analysis of <jats:italic>HYPO<\/jats:italic> exemplifies the complexity and intricacy of evolutionary trajectories of genes within the <jats:styled-content style=\"fixed-case\"><jats:italic>S.\u00a0cerevisiae<\/jats:italic><\/jats:styled-content> pangenome.<\/jats:p><jats:p>To examine possible functions for Hypo, we overexpressed a <jats:italic>HYPO<\/jats:italic>\u2010<jats:italic>GFP<\/jats:italic> fusion protein in both <jats:styled-content style=\"fixed-case\"><jats:italic>S.\u00a0cerevisiae<\/jats:italic><\/jats:styled-content> and <jats:styled-content style=\"fixed-case\"><jats:italic>Saccharomyces<\/jats:italic><\/jats:styled-content> <jats:italic>pastorianus<\/jats:italic>. The protein localised to the plasma membrane where it accumulated initially in distinct foci. Time\u2010lapse fluorescent imaging revealed that when cells are grown in wort, Hypo\u2010gfp fluorescence spreads throughout the membrane during cell growth. The overexpression of Hypo\u2010gfp in <jats:styled-content style=\"fixed-case\"><jats:italic>S.\u00a0cerevisiae<\/jats:italic><\/jats:styled-content> or <jats:italic>S.\u00a0pastorianus<\/jats:italic> strains did not significantly alter cell growth in medium\u2010containing glucose, maltose, maltotriose, or wort at different concentrations.<\/jats:p>","DOI":"10.1002\/yea.3391","type":"journal-article","created":{"date-parts":[[2019,4,9]],"date-time":"2019-04-09T00:38:37Z","timestamp":1554770317000},"page":"425-437","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Evolutionary journey and characterisation of a novel pan\u2010gene associated with beer strains of <scp><i>Saccharomyces cerevisiae<\/i><\/scp>"],"prefix":"10.1002","volume":"36","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7606-6987","authenticated-orcid":false,"given":"Nikolaos","family":"Vakirlis","sequence":"first","affiliation":[{"name":"School 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