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Pseudouridine synthases catalyse pseudouridylation, one of the most prevalent RNA modifications. Pseudouridine synthase 7 modifies a variety of substrates in <jats:italic><jats:styled-content style=\"fixed-case\">Saccharomyces cerevisiae<\/jats:styled-content><\/jats:italic> including tRNA, rRNA, snRNA, and mRNA, but the substrates for other budding yeast Pus7 homologues are not known. We used CRISPR\u2010mediated genome editing to disrupt <jats:styled-content style=\"fixed-case\"><jats:italic>Candida albicans<\/jats:italic><\/jats:styled-content> <jats:italic>PUS7<\/jats:italic> and find absence leads to defects in rRNA processing and a decrease in cell surface hydrophobicity. Furthermore, <jats:styled-content style=\"fixed-case\"><jats:italic>C.\u00a0albicans<\/jats:italic><\/jats:styled-content> Pus7 absence causes temperature sensitivity, defects in filamentation, altered sensitivity to antifungal drugs, and decreased virulence in a wax moth model. In addition, we find <jats:styled-content style=\"fixed-case\"><jats:italic>C.\u00a0albicans<\/jats:italic><\/jats:styled-content> Pus7 modifies tRNA residues, but does not modify a number of other <jats:styled-content style=\"fixed-case\"><jats:italic>S.\u00a0cerevisiae<\/jats:italic><\/jats:styled-content> Pus7 substrates. Our data suggests <jats:styled-content style=\"fixed-case\"><jats:italic>C.\u00a0albicans<\/jats:italic><\/jats:styled-content> Pus7 is important for fungal vigour and may play distinct biological roles than those ascribed to <jats:styled-content style=\"fixed-case\"><jats:italic>S.\u00a0cerevisiae<\/jats:italic><\/jats:styled-content> Pus7.<\/jats:p>","DOI":"10.1002\/yea.3436","type":"journal-article","created":{"date-parts":[[2019,7,31]],"date-time":"2019-07-31T07:24:25Z","timestamp":1564557865000},"page":"669-677","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Pseudouridine synthase 7 impacts <i>Candida albicans<\/i> rRNA processing and morphological plasticity"],"prefix":"10.1002","volume":"36","author":[{"given":"Ethan S.","family":"Pickerill","sequence":"first","affiliation":[{"name":"Department of Biology Ball State University  Muncie IN 47306 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rebecca P.","family":"Kurtz","sequence":"additional","affiliation":[{"name":"Department of Mathematics Ball State University  Muncie IN 47306 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aaron","family":"Tharp","sequence":"additional","affiliation":[{"name":"Department of Biology Ball State University  Muncie IN 47306 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paula","family":"Guerrero Sanz","sequence":"additional","affiliation":[{"name":"Department of Biology Ball State University  Muncie IN 47306 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Munni","family":"Begum","sequence":"additional","affiliation":[{"name":"Department of Mathematics Ball State University  Muncie IN 47306 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8653-8562","authenticated-orcid":false,"given":"Douglas A.","family":"Bernstein","sequence":"additional","affiliation":[{"name":"Department of Biology Ball State University  Muncie IN 47306 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,8,16]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/28.9.1941"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00249a036"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00088a030"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1261\/rna.5520403"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature13802"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/febs.12602"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajhg.2018.10.026"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.01574-07"},{"key":"e_1_2_7_10_1","article-title":"CRISPR\u2010mediated genome editing of the human fungal pathogen Candida albicans","volume":"141","author":"Evans B. 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