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Activation of antioxidant defence system in <jats:styled-content style=\"fixed-case\"><jats:italic>Candida albicans<\/jats:italic><\/jats:styled-content> is known as forefront mechanism to escape drug toxicity. This study evaluated the role of antioxidant defence genes in the susceptibility to fluconazole in <jats:styled-content style=\"fixed-case\"><jats:italic>C. albicans<\/jats:italic><\/jats:styled-content> and also determined the effect of berberine on growth, antioxidant enzymes and the expression of their genes in <jats:styled-content style=\"fixed-case\"><jats:italic>C. albicans<\/jats:italic><\/jats:styled-content> isolates. Expression of major antioxidant genes was significantly increased in fluconazole\u2010resistant isolates in comparison with the susceptible group. Antifungal susceptibility against berberine showed MIC values ranging from 125 to 500 \u03bcg\/ml. Berberine treatment caused upregulation of mRNA expression and enzymatic activities of the targeted major antioxidants. Interestingly, <jats:styled-content style=\"fixed-case\"><jats:italic>C. albicans<\/jats:italic><\/jats:styled-content> exhibited efficient antioxidant response at lower concentrations but could not sufficiently alleviate berberine\u2010induced oxidative stress occurring at concentrations greater than 250 \u03bcg\/ml. Therefore, berberine could serve as a potent Reactive Oxygen Species (ROS)\u2010inducing agent, disrupting the antioxidant system especially in fluconazole\u2010resistant <jats:styled-content style=\"fixed-case\"><jats:italic>C. albicans<\/jats:italic><\/jats:styled-content> to overcome antifungal drug resistance.<\/jats:p><\/jats:sec><jats:sec><jats:title>Take Aways<\/jats:title><jats:p>\n<jats:list list-type=\"bullet\">\n<jats:list-item><jats:p>Evaluated the role of antioxidant enzymes in FLC resistance in <jats:styled-content style=\"fixed-case\"><jats:italic>C. albicans<\/jats:italic><\/jats:styled-content><\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Studied the effect of berberine on growth of different <jats:styled-content style=\"fixed-case\"><jats:italic>C. albicans<\/jats:italic><\/jats:styled-content> isolates<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Investigated the modulation of antioxidant enzymes by berberine in <jats:styled-content style=\"fixed-case\"><jats:italic>C. albicans<\/jats:italic><\/jats:styled-content><\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Studied the effect of berberine on antioxidant gene expression in <jats:styled-content style=\"fixed-case\"><jats:italic>C. albicans<\/jats:italic><\/jats:styled-content><\/jats:p><\/jats:list-item>\n<\/jats:list><\/jats:p><\/jats:sec>","DOI":"10.1002\/yea.3531","type":"journal-article","created":{"date-parts":[[2020,11,3]],"date-time":"2020-11-03T18:39:44Z","timestamp":1604428784000},"page":"157-169","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["Modulation of antioxidant defence system in response to berberine in <scp><i>Candida albicans<\/i><\/scp>"],"prefix":"10.1002","volume":"38","author":[{"given":"Evida","family":"Poopedi","sequence":"first","affiliation":[{"name":"Clinical Microbiology and Infectious Diseases, School of Pathology, Faculty of Health Sciences University of the Witwatersrand  Johannesburg 2193 South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Musa","family":"Marimani","sequence":"additional","affiliation":[{"name":"Department of Anatomical Pathology, School of Pathology, Faculty of Health Sciences University of the Witwatersrand  Johannesburg 2193 South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suliman Yousef","family":"AlOmar","sequence":"additional","affiliation":[{"name":"Doping Research, Department of Zoology, College of Science King Saud University  Riyadh 11451 Kingdom of Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Badr","family":"Aldahmash","sequence":"additional","affiliation":[{"name":"Department of Zoology, College of Science King Saud University  Riyadh 11451 Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2845-0727","authenticated-orcid":false,"given":"Aijaz","family":"Ahmad","sequence":"additional","affiliation":[{"name":"Clinical Microbiology and Infectious Diseases, School of Pathology, Faculty of Health Sciences University of the Witwatersrand  Johannesburg 2193 South Africa"},{"name":"Infection Control, Charlotte Maxeke Johannesburg Academic Hospital National Health Laboratory Service  Johannesburg 2193 South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,12,10]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.2174\/1381612822666160607072748"},{"key":"e_1_2_9_3_1","first-page":"196","article-title":"Superoxide dismutases are involved in Candida albicans biofilm persistence to miconazole","author":"Bink A.","year":"2011","journal-title":"Antimicrob Agents Chemothe"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(76)90527-3"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro1818"},{"key":"e_1_2_9_6_1","unstructured":"Clinical and Laboratory Standards Institute. 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