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In the present study, the synergism of oligostyrylbenzene (OSB) compounds in combination with amphotericin B (AmB) against <jats:styled-content style=\"fixed-case\"><jats:italic>Candida tropicalis<\/jats:italic><\/jats:styled-content> biofilms was investigated. In addition, the toxicity in human blood cells was determined. Synergistic combinations of OSBs and AmB were evaluated to consider future effects of OSBs in vivo. The checkerboard microdilution method was used to study the interactions of one anionic (<jats:bold>1<\/jats:bold>) and two cationic (<jats:bold>2<\/jats:bold> and <jats:bold>3<\/jats:bold>) OSBs with AmB. We investigated the effects of OSBs on reactive oxygen species (ROS) and the levels of the reactive nitrogen intermediates (RNIs). The cellular stress affected biofilm growth through an accumulation of ROS and RNI, at synergistic concentrations of OSBs and AmB. Furthermore, significant surface topography differences were noted upon treatment with the OSB <jats:bold>2<\/jats:bold>\/AmB combination, using confocal laser scanning microscopy in conjunction with the image analysis software COMSTAT. The results revealed a low toxicity to leukocytes and red blood cells at synergistic combinations of cationic OSBs with AmB. These findings demonstrated the antibiofilm effects of OSBs and the synergism of AmB with cationic OSBs against biofilms of <jats:styled-content style=\"fixed-case\"><jats:italic>C.\u00a0tropicalis<\/jats:italic><\/jats:styled-content> for the first time.<\/jats:p>","DOI":"10.1002\/yea.3672","type":"journal-article","created":{"date-parts":[[2021,10,1]],"date-time":"2021-10-01T10:55:15Z","timestamp":1633085715000},"page":"634-645","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Synergic activity of oligostyrylbenzenes with amphotericin B against <scp><i>Candida tropicalis<\/i><\/scp> biofilms"],"prefix":"10.1002","volume":"38","author":[{"given":"Melisa A.","family":"Quinteros","sequence":"first","affiliation":[{"name":"Multidisciplinary Institute of Plant Biology (IMBIV) National Council for Scientific and Technical Research (CONICET)  C\u00f3rdoba Argentina"},{"name":"Deparment of Microbiology, Faculty of Exact, Physical and Natural Sciences National University of C\u00f3rdoba  C\u00f3rdoba Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juan","family":"Tolosa","sequence":"additional","affiliation":[{"name":"Albacete Faculty of Pharmacy, Regional Center for Biomedical Research University of Castilla\u2010La Mancha  Ciudad Real Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joaqu\u00edn C.","family":"Garc\u00eda\u2010Mart\u00ednez","sequence":"additional","affiliation":[{"name":"Albacete Faculty of Pharmacy, Regional Center for Biomedical Research University of Castilla\u2010La Mancha  Ciudad Real Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4836-4842","authenticated-orcid":false,"given":"Paulina L.","family":"P\u00e1ez","sequence":"additional","affiliation":[{"name":"Deparment of Microbiology, Faculty of Exact, Physical and Natural Sciences National University of C\u00f3rdoba  C\u00f3rdoba Argentina"},{"name":"Department of Pharmaceutical Sciences, Faculty of Chemical Sciences National University of C\u00f3rdoba  C\u00f3rdoba Argentina"},{"name":"Research and Development Unit in Pharmaceutical Technology (UNITEFA) National Council for Scientific and Technical Research (CONICET)  C\u00f3rdoba Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8600-1159","authenticated-orcid":false,"given":"Mar\u00eda G.","family":"Paraje","sequence":"additional","affiliation":[{"name":"Multidisciplinary Institute of Plant Biology (IMBIV) National Council for Scientific and Technical Research (CONICET)  C\u00f3rdoba Argentina"},{"name":"Deparment of Microbiology, Faculty of Exact, Physical and Natural Sciences National University of C\u00f3rdoba  C\u00f3rdoba Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,10,27]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.46569-0"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms8121867"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/mmy\/myx143"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.2010.02164.x"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxicon.2013.03.014"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01280-07"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/chem.201303119"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1128\/microbiolspec.MB-0020-2015"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a019729"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1179\/joc.2000.12.6.463"},{"issue":"8","key":"e_1_2_11_12_1","first-page":"4720","article-title":"Effect of the aggregation on the photophysical properties of a blue\u2010emitting star\u2010shaped molecule based on 1,3,5\u2010tristyrylbenzene","volume":"121","author":"Garz\u00f3n A.","year":"2017","journal-title":"The Journal of Physical Chemistry. 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