{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T02:18:21Z","timestamp":1740104301225,"version":"3.37.3"},"reference-count":47,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2022,10,3]],"date-time":"2022-10-03T00:00:00Z","timestamp":1664755200000},"content-version":"vor","delay-in-days":2,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Yeast"],"published-print":{"date-parts":[[2022,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The yeasts, <jats:italic>Saccharomyces pastorianus<\/jats:italic>, are hybrids of <jats:italic>Saccharomyces cerevisiae<\/jats:italic> and <jats:italic>Saccharomyces eubayanus<\/jats:italic> and have acquired traits from the combined parental genomes such as ability to ferment a range of sugars at low temperatures and to produce aromatic flavour compounds, allowing for the production of lager beers with crisp, clean flavours. The polyploid strains are sterile and have reached an evolutionary bottleneck for genetic variation. Here we describe an accelerated evolution approach to obtain lager yeasts with enhanced flavour profiles. As the relative expression of orthologous alleles is a significant contributor to the transcriptome during fermentation, we aimed to induce genetic variation by altering the <jats:italic>S. cerevisiae<\/jats:italic> to <jats:italic>S. eubayanus<\/jats:italic> chromosome ratio. Aneuploidy was induced through the temporary inhibition of the cell's stress response and strains with increased production of aromatic amino acids via the Shikimate pathway were selected by resistance to amino acid analogues. Genomic changes such as gross chromosomal rearrangements, chromosome loss and chromosome gain were detected in the characterised mutants, as were single\u2010nucleotide polymorphisms in <jats:italic>ARO4<\/jats:italic>, encoding for DAHP synthase, the catalytic enzyme in the first step of the Shikimate pathway. Transcriptome analysis confirmed the upregulation of genes encoding enzymes in the Ehrlich pathway and the concomitant increase in the production of higher alcohols and esters such as 2\u2010phenylethanol, 2\u2010phenylethyl acetate, tryptophol, and tyrosol. We propose that the polyploid nature of <jats:italic>S. pastorianus<\/jats:italic> genomes is an advantageous trait supporting opportunities for genetic alteration in otherwise sterile strains.<\/jats:p>","DOI":"10.1002\/yea.3815","type":"journal-article","created":{"date-parts":[[2022,9,21]],"date-time":"2022-09-21T05:10:55Z","timestamp":1663737055000},"page":"535-547","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Enhanced flavour profiles through radicicol induced genomic variation in the lager yeasts, <i>Saccharomyces pastorianus<\/i>"],"prefix":"10.1002","volume":"39","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3868-8552","authenticated-orcid":false,"given":"Roberto","family":"de la Cerda Garcia\u2010Caro","sequence":"first","affiliation":[{"name":"Department of Microbiology, School of Genetics and Microbiology, Moyne Institute Trinity College Dublin  Dublin 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