{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T00:03:36Z","timestamp":1773792216013,"version":"3.50.1"},"reference-count":30,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2023,3,6]],"date-time":"2023-03-06T00:00:00Z","timestamp":1678060800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100007684","name":"Ministry of Education and Science of Ukraine","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100007684","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100014434","name":"Narodowa Agencja Wymiany Akademickiej","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100014434","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Yeast"],"published-print":{"date-parts":[[2023,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The methylotrophic yeast <jats:italic>Komagataella phaffii<\/jats:italic> is considered one of the most effective producers of recombinant proteins of industrial importance. Effective producers should be characterized by the maximal reduction of degradation of the cytosolic recombinant proteins. The mechanisms of degradation of cytosolic proteins in <jats:italic>K. phaffii<\/jats:italic> have not been elucidated; however, data suggest that they are partially degraded in the autophagic pathway. To identify factors that influence this process, a developed system for the selection of recombinant strains of <jats:italic>K. phaffii<\/jats:italic> with impaired autophagic degradation of the heterologous model cytosolic protein (yeast \u03b2\u2010galactosidase) was used for insertional tagging of the genes involved in cytosolic proteins degradation. In one of the obtained strains, the insertion cassette disrupted the open reading frame of the gene encoding \u03b2\u20101,6\u2010N\u2010acetylglucosaminyltransferase. A recombinant strain with deletion of this gene was also obtained. The rate of degradation of the \u03b2\u2010galactosidase enzyme was two times slower in the insertion mutant and 1.5 times slower in the deletion strain as compared to the parental strain with native \u03b2\u20101,6\u2010N\u2010acetylglucosaminyltransferase. The rate of degradation of native <jats:italic>K. phaffii<\/jats:italic> cytosolic and peroxisomal enzymes, formaldehyde dehydrogenase, formate dehydrogenase, and alcohol oxidase, respectively, showed similar trends to that of \u03b2\u2010galactosidase\u2014slower degradation in the deletion and insertional mutants as compared to the wild\u2010type strain, but faster protein degradation relative to the strain completely defective in autophagy. We conclude that <jats:italic>K. phaffii<\/jats:italic> gene designated <jats:italic>ACG1<\/jats:italic>, encoding \u03b2\u20101,6\u2010N\u2010acetylglucosaminyltransferase, is involved in autophagy of the cytosolic and peroxisomal proteins.<\/jats:p>","DOI":"10.1002\/yea.3846","type":"journal-article","created":{"date-parts":[[2023,2,23]],"date-time":"2023-02-23T05:00:05Z","timestamp":1677128405000},"page":"367-376","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["The <i>Komagatella phaffii ACG1<\/i> gene, encoding \u03b2\u20101,6\u2010N\u2010acetylglucosaminyltransferase, is involved in the autophagy of cytosolic and peroxisomal proteins"],"prefix":"10.1002","volume":"40","author":[{"given":"Anastasiya Z.","family":"Zazulya","sequence":"first","affiliation":[{"name":"Department of Molecular Genetics and Biotechnology, Institute of Cell Biology National Academy of Science of Ukraine Lviv 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A.","family":"Sibirny","sequence":"additional","affiliation":[{"name":"Department of Molecular Genetics and Biotechnology, Institute of Cell Biology National Academy of Science of Ukraine Lviv Ukraine"},{"name":"Department of Biotechnology and Microbiology University of Rzeszow Rzeszow Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,3,6]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-10-421"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6976.2000.tb00532.x"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1385\/MB:16:1:23"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.130.2.383"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.3103\/S0095452720050047"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbin.11304"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/ejhg.2009.144"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1385\/0-89603-421-6:81"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/s1567-1356(02)00124-1"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.11.1.255"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M404544200"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.enzmictec.2007.09.008"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msy096"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4020-8292-4_3"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)52451-6"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/yea.1208"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/3527602356.ch15"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0061(200008)16:11<979::aid-yea594>3.0.co;2-c"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12017-018-8482-1"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbiotec.2015.05.007"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.145.1.69"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-94-009-0213-8_34"},{"key":"e_1_2_9_24_1","volume-title":"Molecular 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