{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T11:49:39Z","timestamp":1789213779151,"version":"build-2803163510"},"reference-count":73,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2023,3,14]],"date-time":"2023-03-14T00:00:00Z","timestamp":1678752000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004281","name":"Narodowe Centrum Nauki","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004281","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Yeast"],"published-print":{"date-parts":[[2023,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>One of the initial steps necessary for the development of <jats:italic>Candida<\/jats:italic> infections is the adherence to the host tissues and cells. Recent transcriptomic studies suggest that, in <jats:italic>Candida parapsilosis<\/jats:italic>\u2014a fungal infectious agent that causes systemic candidiasis in immunosuppressed individuals\u2014the adhesion is mediated by pathogen cell\u2010exposed proteins belonging to the agglutinin\u2010like sequence (Als) family. However, to date, the actual interactions of individual members of this family with human cells and extracellular matrix (ECM) have not been characterized in detail. In the current study, we focused attention on two of these <jats:italic>C. parapsilosis<\/jats:italic> Als proteins\u2014CPAR2_404800 and CPAR2_404780\u2014that were proteomically identified in the fungal cell wall of yeasts grown in the media suitable for culturing human epithelial and endothelial cells. Both proteins were extracted from the cell wall and purified, and using a microplate binding assay and a fluorescence microscopic analysis were shown to adhere to human cells of A431 (epithelial) and HMEC\u20101 (endothelial) lines. The human extracellular matrix components that are also plasma proteins\u2014fibronectin and vitronectin\u2014enhanced these interactions, and also could directly bind to CPAR2_404800 and CPAR2_404780 proteins, with a high affinity (<jats:italic>K<\/jats:italic><jats:sub><jats:italic>D<\/jats:italic><\/jats:sub> in a range of 10<jats:sup>\u22127<\/jats:sup> to 10<jats:sup>\u22128<\/jats:sup>\u2009M) as determined by surface plasmon resonance measurements. Our findings highlight the role of proteins CPAR2_404800 and CPAR2_404780 in adhesion to host cells and proteins, contributing to the knowledge of the mechanisms of host\u2010pathogen interactions during <jats:italic>C. parapsilosis<\/jats:italic>\u2010caused infections.<\/jats:p>","DOI":"10.1002\/yea.3847","type":"journal-article","created":{"date-parts":[[2023,3,5]],"date-time":"2023-03-05T05:53:35Z","timestamp":1677995615000},"page":"377-389","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["<i>Candida parapsilosis<\/i> cell wall proteins\u2014CPAR2_404800 and CPAR2_404780\u2014Are adhesins that bind to human epithelial and endothelial cells and extracellular matrix proteins"],"prefix":"10.1002","volume":"40","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6233-6680","authenticated-orcid":false,"given":"Dorota","family":"Satala","sequence":"first","affiliation":[{"name":"Department of Comparative Biochemistry and Bioanalytics, Faculty of Biochemistry, Biophysics and Biotechnology Jagiellonian University in Krakow Krakow Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6070-6919","authenticated-orcid":false,"given":"Justyna","family":"Karkowska\u2010Kuleta","sequence":"additional","affiliation":[{"name":"Department of Comparative Biochemistry and Bioanalytics, Faculty of Biochemistry, Biophysics and Biotechnology Jagiellonian University in Krakow Krakow Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1814-9263","authenticated-orcid":false,"given":"Grazyna","family":"Bras","sequence":"additional","affiliation":[{"name":"Department of Comparative Biochemistry and Bioanalytics, Faculty of Biochemistry, Biophysics and Biotechnology Jagiellonian University in Krakow Krakow Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3225-7391","authenticated-orcid":false,"given":"Maria","family":"Rapala\u2010Kozik","sequence":"additional","affiliation":[{"name":"Department of Comparative Biochemistry and Bioanalytics, Faculty of Biochemistry, Biophysics and Biotechnology Jagiellonian University in Krakow Krakow Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4935-4112","authenticated-orcid":false,"given":"Andrzej","family":"Kozik","sequence":"additional","affiliation":[{"name":"Department of Analytical Biochemistry, Faculty of Biochemistry, Biophysics and Biotechnology Jagiellonian University in Krakow Krakow Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,3,14]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-019-42517-y"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1128\/EC.00340-06"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10456-009-9136-6"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-019-40771-8"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1080\/21505594.2015.1112491"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1128\/mSphere.01071-20"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature08064"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.62.1.130-180.1998"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/cmi.12002"},{"issue":"10","key":"e_1_2_9_11_1","first-page":"4177","article-title":"Up\u2010regulation of fibronectin and tissue transglutaminase promotes cell invasion involving increased association with integrin and MMP expression in A431 cells","volume":"30","author":"Chen S. 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