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Mother\u2010to\u2010child transmission is the main intrafamilial route of <jats:italic>H. pylori<\/jats:italic> transmission. However, how it transmits from mother to child is still being determined. Vaginal yeast often transmits to neonates during delivery. Therefore, <jats:italic>H. pylori<\/jats:italic> hosted in yeast might follow the same transmission route. This study aimed to detect intracellular <jats:italic>H. pylori<\/jats:italic> in vaginal and fecal yeasts isolates and explore the role of yeast in <jats:italic>H. pylori<\/jats:italic> transmission. Yeast was isolated from the mothers' vaginal discharge and neonates' feces and identified by internal transcribed spacer (ITS) sequencing. <jats:italic>H. pylori<\/jats:italic> 16S rRNA and antigen were detected in yeast isolates by polymerase chain reaction and direct immunofluorescence assay. Genetic relationships of <jats:italic>Candida<\/jats:italic> strains isolated from seven mothers and their corresponding neonates were determined by random amplified polymorphic DNA (RAPD) fingerprinting and ITS alignment. The <jats:italic>Candida<\/jats:italic> isolates from four mother\u2013neonate pairs had identical RAPD patterns and highly homologous ITS sequences. The current study showed <jats:italic>H. pylori<\/jats:italic> could be sheltered within yeast colonizing the vagina, and fecal yeast from neonates is genetically related to the vaginal yeast from their mothers. Thus, vaginal yeast presents a potential reservoir of <jats:italic>H. pylori<\/jats:italic> and plays a vital role in the transmission from mother to neonate.<\/jats:p>","DOI":"10.1002\/yea.3891","type":"journal-article","created":{"date-parts":[[2023,8,10]],"date-time":"2023-08-10T11:55:19Z","timestamp":1691668519000},"page":"401-413","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Intracellular presence and genetic relationship of <i>Helicobacter pylori<\/i> within neonates' fecal yeasts and their mothers' vaginal yeasts"],"prefix":"10.1002","volume":"40","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3015-2932","authenticated-orcid":false,"given":"Tingxiu","family":"Yang","sequence":"first","affiliation":[{"name":"Key Laboratory of Microbiology and Parasitology of Education Department of Guizhou &amp; Joint Laboratory of Helicobacter Pylori and Intestinal Microecology of Affiliated Hospital of Guizhou 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Guiyang China"},{"name":"Key Laboratory of Endemic and Ethnic Diseases of Ministry of Education &amp; Key Laboratory of Environmental Pollution Monitoring and Disease Control, Ministry of Education Guizhou Medical University Guiyang China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,8,10]]},"reference":[{"issue":"4","key":"e_1_2_9_2_1","first-page":"487","article-title":"Helicobacter pylori infection in amniotic fluid may cause hyperemesis gravidarum","volume":"93","author":"Ayd\u0131n M.","year":"2020","journal-title":"The Yale Journal of Biology and 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