{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T22:19:43Z","timestamp":1780525183671,"version":"3.54.1"},"reference-count":19,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2024,8,9]],"date-time":"2024-08-09T00:00:00Z","timestamp":1723161600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Yeast"],"published-print":{"date-parts":[[2024,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The CRISPR\u2010Cas9 system is extremely useful for genome editing in many species, including the model yeast <jats:italic>Saccharomyces cerevisiae<\/jats:italic>, and other yeast species. We have previously reported the use of an inducible CRISPR\u2010Cas9 system in <jats:italic>Candida glabrata<\/jats:italic>, which allows genome editing but also the study of double\u2010strand break (DSB) repair. We report, in this study, a comparable system for <jats:italic>C. glabrata<\/jats:italic>, relying on a new plasmid, which is more stable than the previous one. We also report the use of this plasmid to induce DSBs in two additional human pathogens, <jats:italic>Candida bracarensis<\/jats:italic> and <jats:italic>Candida nivariensis.<\/jats:italic> We examine lethality induced by an in vivo DSB in the three species and describe the different types of nonhomologous end\u2010joining (NHEJ) events detected in these three pathogens.<\/jats:p>","DOI":"10.1002\/yea.3976","type":"journal-article","created":{"date-parts":[[2024,8,10]],"date-time":"2024-08-10T06:43:30Z","timestamp":1723272210000},"page":"560-567","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["In vivo CRISPR\u2010Cas9 expression in <i>Candida glabrata<\/i>, <i>Candida bracarensis<\/i>, and <i>Candida nivariensis<\/i>: A versatile tool to study chromosomal break repair"],"prefix":"10.1002","volume":"41","author":[{"given":"Killian","family":"M\u00e9tivier","sequence":"first","affiliation":[{"name":"GQE\u2010Le Moulon, IDEEV Universit\u00e9 Paris\u2010Saclay, CNRS, INRAE, AgroPariTech Gif\u2010sur\u2010Yvette France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Youfang","family":"Zhou\u2010Li","sequence":"additional","affiliation":[{"name":"GQE\u2010Le Moulon, IDEEV Universit\u00e9 Paris\u2010Saclay, CNRS, INRAE, AgroPariTech Gif\u2010sur\u2010Yvette France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0456-0377","authenticated-orcid":false,"given":"C\u00e9cile","family":"Fairhead","sequence":"additional","affiliation":[{"name":"GQE\u2010Le Moulon, IDEEV Universit\u00e9 Paris\u2010Saclay, CNRS, INRAE, AgroPariTech Gif\u2010sur\u2010Yvette France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,8,9]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1101\/pdb.prot4148"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/cpz1.647"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0140990"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fgb.2017.08.004"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/genetics\/61.2.377"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature02579"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep35766"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-14-623"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/yea.320110408"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aat5011"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1716855115"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/yea.3440"},{"key":"e_1_2_8_14_1","first-page":"71","volume-title":"Candida: Comparative and functional genomics","author":"Muller H.","year":"2007"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1128\/EC.00456\u201007"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1098\/rsob.120093"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-013-2009-3"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1128\/mSphere.00154-18"},{"key":"e_1_2_8_19_1","doi-asserted-by":"crossref","unstructured":"Yarrington R. M. Verma S. Schwartz S. Trautman J. K. &Carroll D.(2018).Nucleosomes inhibit target cleavage by CRISPR\u2010Cas9in vivo.Proceedings of the National Academy of Sciences of the United States of America 115 9351\u20139358.https:\/\/doi.org\/10.1073\/pnas.1810062115","DOI":"10.1073\/pnas.1810062115"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1534\/g3.113.006908"}],"container-title":["Yeast"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/yea.3976","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,9,9]],"date-time":"2024-09-09T09:22:47Z","timestamp":1725873767000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/yea.3976"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,8,9]]},"references-count":19,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2024,9]]}},"alternative-id":["10.1002\/yea.3976"],"URL":"https:\/\/doi.org\/10.1002\/yea.3976","archive":["Portico"],"relation":{},"ISSN":["0749-503X","1097-0061"],"issn-type":[{"value":"0749-503X","type":"print"},{"value":"1097-0061","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,8,9]]},"assertion":[{"value":"2024-05-22","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-07-27","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-08-09","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}