{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,9]],"date-time":"2026-08-09T23:46:18Z","timestamp":1786319178461,"version":"build-2736575974"},"reference-count":63,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2024,9,11]],"date-time":"2024-09-11T00:00:00Z","timestamp":1726012800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Yeast"],"published-print":{"date-parts":[[2024,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Polyphosphate (polyP) is an intriguing molecule that is found in almost any organism, covering a multitude of cellular functions. In industry, polyP is used due to its unique physiochemical properties, including pH buffering, water binding, and bacteriostatic activities. Despite the importance of polyP, its analytics is still challenging, with the gold standard being <jats:sup>31<\/jats:sup>P NMR. Here, we present a simple staining method using the fluorescent dye JC\u2010D7 for the semi\u2010quantitative polyP evaluation in yeast extracts. Notably, fluorescence response was affected by polyP concentration and polymer chain length in the 0.5\u2013500\u2009\u00b5g\/mL polyP concentration range. Hence, for polyP samples of unknown chain compositions, JC\u2010D7 cannot be used for absolute quantification. Fluorescence of JC\u2010D7 was unaffected by inorganic phosphate up to 50\u2009mM. Trace elements (FeSO<jats:sub>4<\/jats:sub>\u2009&gt;\u2009CuSO<jats:sub>4<\/jats:sub>\u2009&gt;\u2009CoCl<jats:sub>2<\/jats:sub>\u2009&gt;\u2009ZnSO<jats:sub>4<\/jats:sub>) and toxic mineral salts (PbNO<jats:sub>3<\/jats:sub> and HgCl<jats:sub>2<\/jats:sub>) diminished polyP\u2013induced JC\u2010D7 fluorescence, affecting its applicability to samples containing polyP\u2013metal complexes. The fluorescence was only marginally affected by other parameters, such as pH and temperature. After validation, this simple assay was used to elucidate the degree of polyP production by yeast strains carrying gene deletions in (poly)phosphate homeostasis. The results suggest that staining with JC\u2010D7 provides a robust and sensitive method for detecting polyP in yeast extracts and likely in extracts of other microbes. The simplicity of the assay enables high\u2010throughput screening of microbes to fully elucidate and potentially enhance biotechnological polyP production, ultimately contributing to a sustainable phosphorus utilization.<\/jats:p>","DOI":"10.1002\/yea.3979","type":"journal-article","created":{"date-parts":[[2024,9,12]],"date-time":"2024-09-12T04:54:31Z","timestamp":1726116871000},"page":"593-604","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Rapid Fluorescence Assay for Polyphosphate in Yeast Extracts Using JC\u2010D7"],"prefix":"10.1002","volume":"41","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4842-5230","authenticated-orcid":false,"given":"Alexander","family":"Deitert","sequence":"first","affiliation":[{"name":"Institute of Applied Microbiology\u2014iAMB, Aachen Biology and Biotechnology\u2014ABBt RWTH Aachen University Aachen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7367-7651","authenticated-orcid":false,"given":"Jana","family":"Fees","sequence":"additional","affiliation":[{"name":"Institute of Applied Microbiology\u2014iAMB, Aachen Biology and Biotechnology\u2014ABBt RWTH Aachen University Aachen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2119-1913","authenticated-orcid":false,"given":"Anna","family":"Mertens","sequence":"additional","affiliation":[{"name":"Institute of Applied Microbiology\u2014iAMB, Aachen Biology and Biotechnology\u2014ABBt RWTH Aachen University Aachen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Duc","family":"Nguyen Van","sequence":"additional","affiliation":[{"name":"Department of Food Chemistry and Toxicology, Institute of Food Technology and Food Chemistry Technische Universit\u00e4t Berlin Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9140-2448","authenticated-orcid":false,"given":"Maria","family":"Maares","sequence":"additional","affiliation":[{"name":"Department of Food Chemistry and Toxicology, Institute of Food Technology and Food Chemistry Technische Universit\u00e4t Berlin Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1622-8718","authenticated-orcid":false,"given":"Hajo","family":"Haase","sequence":"additional","affiliation":[{"name":"Department of Food Chemistry and Toxicology, Institute of Food Technology and Food Chemistry Technische Universit\u00e4t Berlin Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0961-4976","authenticated-orcid":false,"given":"Lars Mathias","family":"Blank","sequence":"additional","affiliation":[{"name":"Institute of Applied Microbiology\u2014iAMB, Aachen Biology and Biotechnology\u2014ABBt RWTH Aachen University Aachen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0317-0905","authenticated-orcid":false,"given":"Claudia","family":"Keil","sequence":"additional","affiliation":[{"name":"Department of Food Chemistry and Toxicology, Institute of Food Technology and Food Chemistry Technische Universit\u00e4t Berlin Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,9,11]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/hlca.19770600115"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-319-41073-9_2"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1021\/cb5000696"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10895-008-0315-4"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/1751-7915.14250"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1042\/BST20230483"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1567-1364.2012.00812.x"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0406909101"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.15698\/mic2017.01.551"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/mmi.13396"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1128\/mcb.11.6.3229-3238.1991"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ab.2018.02.018"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ab.2018.09.021"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1093\/femsyr\/foz011"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/bit.27337"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.9b00567"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.9b05144"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi2014954"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.btre.2016.04.003"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.copbio.2024.103107"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1021\/es100191h"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1742-4658.2009.07524.x"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/bit.28274"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep21760"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.4081\/ejh.2013.e34"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0158835"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-020-60263-4"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1039\/c3mt00103b"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccr.2021.213810"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1177\/38.9.1696951"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.3109\/10520299509108199"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.geoderma.2022.116281"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20140237"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11274-018-2523-7"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.procbio.2011.10.028"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1128\/mcb.5.8.2131-2141.1985"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M111.266320"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.02592-12"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.chemrev.9b00460"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.1039\/c9mt00104b"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.1186\/1475-2859-11-36"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.11.12.4309"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.15.2.997"},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ab.2008.08.002"},{"key":"e_1_2_10_46_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.est.1c04782"},{"key":"e_1_2_10_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.femsyr.2005.03.002"},{"key":"e_1_2_10_48_1","doi-asserted-by":"publisher","DOI":"10.1111\/jpy.13145"},{"key":"e_1_2_10_49_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.68.5.2095-2100.2002"},{"key":"e_1_2_10_50_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.biochem.77.083007.093039"},{"key":"e_1_2_10_51_1","doi-asserted-by":"publisher","DOI":"10.1080\/02603594.2021.1973444"},{"key":"e_1_2_10_52_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.71.10.5692-5701.2005"},{"key":"e_1_2_10_53_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2020.00938"},{"key":"e_1_2_10_54_1","doi-asserted-by":"publisher","DOI":"10.2147\/IJN.S389819"},{"key":"e_1_2_10_55_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.151269398"},{"key":"e_1_2_10_56_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.meatsci.2009.09.003"},{"key":"e_1_2_10_57_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.1990.tb03931.x"},{"key":"e_1_2_10_58_1","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms10091746"},{"key":"e_1_2_10_59_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1010298"},{"key":"e_1_2_10_60_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja01158a004"},{"key":"e_1_2_10_61_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsomega.2c07642"},{"key":"e_1_2_10_62_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.est.4c04545"},{"key":"e_1_2_10_63_1","doi-asserted-by":"publisher","DOI":"10.2903\/j.efsa.2019.5674"},{"key":"e_1_2_10_64_1","doi-asserted-by":"publisher","DOI":"10.1111\/tpj.14642"}],"container-title":["Yeast"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/yea.3979","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,10,9]],"date-time":"2024-10-09T11:59:48Z","timestamp":1728475188000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/yea.3979"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,9,11]]},"references-count":63,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2024,10]]}},"alternative-id":["10.1002\/yea.3979"],"URL":"https:\/\/doi.org\/10.1002\/yea.3979","archive":["Portico"],"relation":{},"ISSN":["0749-503X","1097-0061"],"issn-type":[{"value":"0749-503X","type":"print"},{"value":"1097-0061","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,9,11]]},"assertion":[{"value":"2024-03-15","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-08-23","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-09-11","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}