{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T07:29:22Z","timestamp":1792654162004,"version":"4.5.0"},"reference-count":12,"publisher":"Wiley","issue":"5-7","license":[{"start":{"date-parts":[[2025,3,15]],"date-time":"2025-03-15T00:00:00Z","timestamp":1741996800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Yeast"],"published-print":{"date-parts":[[2025,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>The Lithium\u2013PEG method for transforming yeast cells is a standard procedure used in most yeast laboratories. After several optimizations, this method can yield up to 10<jats:sup>6<\/jats:sup> transformants per \u00b5g of plasmid. Some applications, such as library screening or complex transformations, necessitate maximizing transformation yield. Here, we demonstrate that the addition of a sorbitol solution serves as an osmo\u2010protectant during and after heat shock, resulting in up to a tenfold increase in transformation efficiency. This optimization requires only one additional pipetting step compared to the original protocol, making it practical for routine use.<\/jats:p>","DOI":"10.1002\/yea.3999","type":"journal-article","created":{"date-parts":[[2025,3,15]],"date-time":"2025-03-15T05:30:06Z","timestamp":1742016606000},"page":"109-115","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Enhancing Yeast Transformation: Achieving up to a Tenfold Increase Through a Single Adjustment in the Lithium Acetate\u2013Polyethylene Glycol Method"],"prefix":"10.1002","volume":"42","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-9976-7098","authenticated-orcid":false,"given":"Mathilde","family":"Kadouch","sequence":"first","affiliation":[{"name":"Univ. Bordeaux, CNRS, Bordeaux INP, CBMN, UMR 5248 Pessac France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pierre","family":"Gaspin","sequence":"additional","affiliation":[{"name":"Univ. Bordeaux, CNRS, Bordeaux INP, CBMN, UMR 5248 Pessac France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christelle","family":"Marchal","sequence":"additional","affiliation":[{"name":"Univ. Bordeaux, CNRS, Bordeaux INP, CBMN, UMR 5248 Pessac France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sabine","family":"Castano","sequence":"additional","affiliation":[{"name":"Univ. Bordeaux, CNRS, Bordeaux INP, CBMN, UMR 5248 Pessac France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4110-4677","authenticated-orcid":false,"given":"Christophe","family":"Cullin","sequence":"additional","affiliation":[{"name":"Univ. Bordeaux, CNRS, Bordeaux INP, CBMN, UMR 5248 Pessac France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,3,15]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/yea.1502"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/275104a0"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.talanta.2008.06.018"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/20.6.1425"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2007.13"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2007.15"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/yea.320110408"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1128\/jb.153.1.163-168.1983"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.4161\/bbug.1.6.13257"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.91.2.766"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00294-011-0341-7"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00340712"}],"container-title":["Yeast"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/yea.3999","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,7,17]],"date-time":"2025-07-17T12:31:37Z","timestamp":1752755497000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/yea.3999"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,3,15]]},"references-count":12,"journal-issue":{"issue":"5-7","published-print":{"date-parts":[[2025,6]]}},"alternative-id":["10.1002\/yea.3999"],"URL":"https:\/\/doi.org\/10.1002\/yea.3999","archive":["Portico"],"relation":{},"ISSN":["0749-503X","1097-0061"],"issn-type":[{"value":"0749-503X","type":"print"},{"value":"1097-0061","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,3,15]]},"assertion":[{"value":"2024-09-11","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-03-04","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-03-15","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}