{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,2]],"date-time":"2026-01-02T09:35:32Z","timestamp":1767346532918,"version":"3.48.0"},"reference-count":21,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2025,11,6]],"date-time":"2025-11-06T00:00:00Z","timestamp":1762387200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Yeast"],"published-print":{"date-parts":[[2025,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Saccharomyces cerevisiae<\/jats:italic>\n                    yeast cells have been shown to produce 18S and 25S ribosomal RNA molecules that are resistant to degradation by exonucleases, which require a 5\u2032 monophosphate for activity. These resistant RNA species accumulate during the diauxic shift, a phase marked by reduced TOR signaling. To further investigate the link between TOR activity and the accumulation of resistant rRNA, we examined the effects of pharmacological TOR inhibition. Treatment with rapamycin, an active TOR suppressor, led to increased levels of resistant 18S and 25S RNA. Importantly, this accumulation was also observed in cells with constitutively active RNA polymerase I (CARA), indicating that the resistant RNA species arise independently of RNA Pol I transcriptional regulation. Similarly, a TOR1\u2010deleted mutant of\n                    <jats:italic>Saccharomyces cerevisiae<\/jats:italic>\n                    produces resistant 18S and 25S rRNA species in a sustained manner. Thiouracil labeling revealed that rRNA molecules generated during the logarithmic growth phase can be converted into the resistant form, suggesting a posttranscriptional modification process. Furthermore, thiouracil uptake assays demonstrated that overall rRNA synthesis decreases during the diauxic phase. Notably, decapping of the resistant rRNAs restored their sensitivity to exonucleases, indicating that the resistance is conferred by 5\u2032 end modifications, likely involving the addition of one or more phosphate groups.\n                  <\/jats:p>","DOI":"10.1002\/yea.70007","type":"journal-article","created":{"date-parts":[[2025,11,6]],"date-time":"2025-11-06T12:51:20Z","timestamp":1762433480000},"page":"273-282","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["18S and 25S Exonuclease Resistant Ribosomal RNA Molecules Are Produced by 5\u2032\u2010End Modification During TOR Inhibition."],"prefix":"10.1002","volume":"42","author":[{"given":"Miguel A.","family":"Rocha","sequence":"first","affiliation":[{"name":"Research Division Greater Los Angeles VA Healthcare System California Los Angeles USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gowda","family":"Bhavani","sequence":"additional","affiliation":[{"name":"Research Division Greater Los Angeles VA Healthcare System California Los Angeles USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jacob","family":"Fleischmann","sequence":"additional","affiliation":[{"name":"Research Division Greater Los Angeles VA Healthcare System California Los Angeles USA"},{"name":"Department of Medicine David Geffen School of Medicine at UCLA California Los Angeles USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,11,6]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.21.15.4847-4855.2001"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.4161\/cc.6.1.3649"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmolb.2024.1395220"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12867-018-0102-y"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12860-020-00303-z"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/lary.23401"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.260378"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.3390\/biom13040642"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/wrna.1269"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0909051106"},{"key":"e_1_2_11_12_1","first-page":"229","article-title":"Labeling of Eukaryotic Messenger RNA 5\u02b9 Terminus With Phosphorus \u201032: Use of Tobacco Acid Pyrophosphatase for Removal of Cap Structures","volume":"2","author":"Lockard R. 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