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For example,\n                    <jats:italic>N. glabratus<\/jats:italic>\n                    (formerly known as\n                    <jats:italic>Candida glabrata<\/jats:italic>\n                    ) is a common human pathogen, whereas\n                    <jats:italic>N. bracarensis<\/jats:italic>\n                    and\n                    <jats:italic>N. nivariensis<\/jats:italic>\n                    have been isolated in clinical settings but are not common human pathogens.\n                    <jats:italic>N. delphensis<\/jats:italic>\n                    was isolated from fruit and there is no evidence it is pathogenic. Given the differences, we developed the clade as a molecular genetic system where we could introduce plasmids and assess transcriptional output from cloned promoters. We engineered a CRISPR\/Cas9 plasmid that allows for rapid Gibson cloning of gRNAs, generated auxotrophic strains for amino acids and nucleotides, and introduced plasmids into each species. We used promoter\u2010YFP plasmids to determine that while there are differences between the species, each species likely has intact thiamine and phosphate (THI and PHO) signal transduction pathways, and that gene expression in\n                    <jats:italic>N. glabratus<\/jats:italic>\n                    and\n                    <jats:italic>N. bracarensis<\/jats:italic>\n                    is more similar to one another than to the other two species. Finally, we determine that\n                    <jats:italic>N. glabratus<\/jats:italic>\n                    ,\n                    <jats:italic>N. bracarensis<\/jats:italic>\n                    , and\n                    <jats:italic>N. nivariensis<\/jats:italic>\n                    persist in a murine macrophage for 24\u2009h, whereas\n                    <jats:italic>N. delphensis<\/jats:italic>\n                    does not. This work describes new molecular tools for genetic manipulation in the\n                    <jats:italic>Nakaseomyces<\/jats:italic>\n                    clade and allows for evolutionary questions to be explored.\n                  <\/jats:p>","DOI":"10.1002\/yea.70029","type":"journal-article","created":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T10:22:58Z","timestamp":1780482178000},"page":"202-213","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Genetic Tools in the\n                    <i>Nakaseomyces<\/i>\n                    clade for Evolutionary Comparisons of Signal Transduction Pathways"],"prefix":"10.1002","volume":"43","author":[{"given":"Maria","family":"Abraham","sequence":"first","affiliation":[{"name":"Department of Biology Villanova University Villanova Pennsylvania USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christine L.","family":"Iosue","sequence":"additional","affiliation":[{"name":"Department of Biology Villanova University Villanova Pennsylvania USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dennis D.","family":"Wykoff","sequence":"additional","affiliation":[{"name":"Department of Biology Villanova University Villanova Pennsylvania USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,6,3]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/femsyr\/fov114"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsinfecdis.4c00477"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/mmy\/myae041"},{"key":"e_1_2_9_5_1","doi-asserted-by":"crossref","unstructured":"Corrigan M. W. C. L.Kerwin\u2010Iosue A. S.Kuczmarski K. B.Amin andD. D.Wykoff.2013. The Fate of Linear DNA in Saccharomyces cerevisiae and Candida glabrata: The Role of Homologous and Non\u2010Homologous End Joining Fairhead C editor. 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Life\u2010History Strategies and Carbon Metabolism Gene Dosage in the Nakaseomyces Yeasts Nielsen J editor. 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