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Although\n                    <jats:italic>Saccharomyces cerevisiae<\/jats:italic>\n                    remains the dominant industrial yeast, its metabolic constraints and stress sensitivity highlight the need for alternative microbial chassis. Environmental selection shapes microbial phenotypes, and ecologically diverse isolates represent an underexplored reservoir of industrially relevant traits. Here, we established the Brazilian Yeast Collection (BRYC), comprising 79 isolates spanning 18 genera and 28 named species collected across Brazilian biomes, and systematically characterized their phenotypic diversity under 19 industrially relevant conditions\u2014including carbon sources, biomass\u2010derived inhibitors, elevated temperatures, and high ethanol concentrations\u2014as well as performance on biomass feedstock for industrial bioenergy applications. Across 19 conditions, an industrial\n                    <jats:italic>S. cerevisiae<\/jats:italic>\n                    control ranked first in only one (14% ethanol) despite the panel including strains selected or engineered for low pH tolerance, aldehyde tolerance, xylose utilization, and industrial fermentation performance. 68% of BRYC isolates also exceeded the industrial controls in growth rate robustness. Twenty isolates ranked in the top 10 across three or more conditions, with\n                    <jats:italic>Wickerhamomyces anomalus<\/jats:italic>\n                    (BRYC03 and BRYC30),\n                    <jats:italic>Pichia kudriavzevii<\/jats:italic>\n                    (BRYC98), and\n                    <jats:italic>Sungouiella flosculorum<\/jats:italic>\n                    (BRYC21) emerging as the most versatile performers. Stress\u2010tolerance profiles predicted performance on complex industrial substrates, supporting condition\u2010relevant phenotyping as a framework for rational strain selection. These rankings were, however, substantially format\u2010dependent, with low concordance between liquid and solid cultivation across most conditions (median top\u201010 overlap = 0.20), underscoring the importance of matching cultivation format to target industrial processes. These findings expand the portfolio of stress\u2010resilient, metabolically versatile yeasts and provide a foundation for their development as next\u2010generation industrial fermenters.\n                  <\/jats:p>","DOI":"10.1002\/yea.70045","type":"journal-article","created":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T11:58:42Z","timestamp":1790251122000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Screening of Brazilian Isolated Yeasts Reveals Multiple Potential Chassis for Industrial Biotechnology"],"prefix":"10.1002","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2213-1899","authenticated-orcid":false,"given":"Giovanna R.","family":"Maklouf","sequence":"first","affiliation":[{"name":"Laboratory of Genomics and Bioenergy (LGE) Institute of Biology, University of Campinas (Unicamp) Campinas Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6701-3450","authenticated-orcid":false,"given":"Lara I. S.","family":"Sousa","sequence":"additional","affiliation":[{"name":"Laboratory of Genomics and Bioenergy (LGE) Institute of Biology, University of Campinas (Unicamp) Campinas Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5805-2610","authenticated-orcid":false,"given":"Juliana Pimentel","family":"Galhardo","sequence":"additional","affiliation":[{"name":"Laboratory of Genomics and Bioenergy (LGE) Institute of Biology, University of Campinas (Unicamp) Campinas Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4860-9790","authenticated-orcid":false,"given":"Cecilia","family":"Trivellin","sequence":"additional","affiliation":[{"name":"Department of Organismic and Evolutionary Biology Harvard University Cambridge Massachusetts USA"},{"name":"Department of Chemistry and Molecular Biology Gothenburg University Gothenburg Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-6624-3416","authenticated-orcid":false,"given":"Jo\u00e3o Pedro R.","family":"Prado","sequence":"additional","affiliation":[{"name":"Laboratory of Genomics and Bioenergy (LGE) Institute of Biology, University of Campinas (Unicamp) Campinas Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6991-6322","authenticated-orcid":false,"given":"T\u00e1ssia Cristina da","family":"Silva","sequence":"additional","affiliation":[{"name":"Laboratory of Genomics and Bioenergy (LGE) Institute of Biology, University of Campinas (Unicamp) Campinas Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3126-7923","authenticated-orcid":false,"given":"Ana C. S. R.","family":"de Carvalho","sequence":"additional","affiliation":[{"name":"Laboratory of Bees Biotechnology and Sustainability Studies, University of S\u00e3o Paulo (USP) S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8259-6519","authenticated-orcid":false,"given":"Juliana","family":"Jos\u00e9","sequence":"additional","affiliation":[{"name":"Laboratory of Genetics and Molecular Cardiology (LGCM), InCor, HC\u2010FMUSP University of S\u00e3o Paulo (USP) S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4140-3482","authenticated-orcid":false,"given":"Gon\u00e7alo A. 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