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However, successful domestication requires not only efficient sugar utilization but also enhanced tolerance to the stresses associated with industrial fermentation, particularly ethanol accumulation, osmotic pressure, and oxidative conditions. To investigate stress responses in\n                    <jats:italic>Sp. passalidarum<\/jats:italic>\n                    , its repertoire of transcription factors and over 300 stress\u2010related proteins were predicted and compared to orthologs in\n                    <jats:italic>Saccharomyces cerevisiae<\/jats:italic>\n                    . While most stress\u2010related proteins were conserved between the two species, more than 70% of\n                    <jats:italic>Sp. passalidarum<\/jats:italic>\n                    transcription factors showed less than 30% sequence identity, lacked recognizable domains, or diverged within functional domains. Gene complementation assays showed that the\n                    <jats:italic>Sp. passalidarum YAP1<\/jats:italic>\n                    and\n                    <jats:italic>MSN\u2010like<\/jats:italic>\n                    genes showed limited or incomplete complementation of oxidative stress tolerance in\n                    <jats:italic>S. cerevisiae<\/jats:italic>\n                    mutant strains compared with the corresponding native genes. Together, these findings support functional divergence of the analyzed transcription factors in the heterologous\n                    <jats:italic>S. cerevisiae<\/jats:italic>\n                    system and suggest that regulatory divergence may contribute to stress\u2010response differences between\n                    <jats:italic>Spathaspora passalidarum<\/jats:italic>\n                    and\n                    <jats:italic>Saccharomyces cerevisiae<\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.1002\/yea.70046","type":"journal-article","created":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T13:36:50Z","timestamp":1789738610000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Comparative Genomics and Heterologous Complementation Reveal Sequence and Structural Divergence of Selected Transcription Factors From\n                    <i>Spathaspora passalidarum<\/i>"],"prefix":"10.1002","author":[{"given":"Fernanda Matias","family":"Albuini","sequence":"first","affiliation":[{"name":"Departamento de Bioqu\u00edmica e Biologia Molecular Universidade Federal de Vi\u00e7osa Vi\u00e7osa Minas Gerais Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5999-5805","authenticated-orcid":false,"given":"Alex Gazolla","family":"de Castro","sequence":"additional","affiliation":[{"name":"Departamento de Bioqu\u00edmica e Biologia Molecular Universidade Federal de Vi\u00e7osa Vi\u00e7osa Minas Gerais Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L\u00edlian Em\u00eddio","family":"Ribeiro","sequence":"additional","affiliation":[{"name":"Departamento de Bioqu\u00edmica e Biologia Molecular Universidade Federal de Vi\u00e7osa Vi\u00e7osa Minas Gerais Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lizandra Cristina","family":"de Oliveira Figueiredo Gazolla","sequence":"additional","affiliation":[{"name":"Departamento de Bioqu\u00edmica e Biologia Molecular Universidade Federal de Vi\u00e7osa Vi\u00e7osa Minas Gerais Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0056-9075","authenticated-orcid":false,"given":"Carlos Augusto","family":"Rosa","sequence":"additional","affiliation":[{"name":"Departamento de Microbiologia, Instituto de Ci\u00eancias Biol\u00f3gicas Universidade Federal de Minas Gerais Belo Horizonte Minas Gerais Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tiago Ant\u00f4nio","family":"de Oliveira Mendes","sequence":"additional","affiliation":[{"name":"Departamento de Bioqu\u00edmica e Biologia Molecular Universidade Federal de Vi\u00e7osa Vi\u00e7osa Minas Gerais Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2674-6471","authenticated-orcid":false,"given":"Luciano Gomes","family":"Fietto","sequence":"additional","affiliation":[{"name":"Departamento de Bioqu\u00edmica e Biologia Molecular Universidade Federal de Vi\u00e7osa Vi\u00e7osa Minas Gerais Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,18]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-023-12730-x"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1131969"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0022-2836(05)80360-2"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbiosc.2017.03.009"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1534\/genetics.106.062794"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/yea.3279"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s42770-022-00693-6"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrg1990"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13068-015-0418-5"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13068-019-1360-8"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1093\/femsyr\/fox036"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gky995"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-6445(00)00035-8"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/0471250953.bi0612s35.Using"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2018.03264"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2007.13"},{"issue":"80","key":"e_1_2_10_18_1","first-page":"563","article-title":"Life With 6000 Genes Conveniently Among the Different Interna\u2010 Old Questions and New Answers The Genome. 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