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This system was used to analyse hypoxia\u2010dependent <jats:italic>PsADH2<\/jats:italic> regulation. Reporter gene activity was only found under oxygen limitation on a fermentable carbon source. The promoter was not induced by oxygen limitation in the Crabtree\u2010positive yeast <jats:italic>Saccharomyces cerevisiae<\/jats:italic>. Promoter deletions revealed that a region of 15 bp contained the essential site for hypoxic induction. This motif was different from the known hypoxia response elements of <jats:italic>S. cerevisiae<\/jats:italic> but showed some similarity to the mammalian HIF\u20101 binding site. Electrophoretic mobility shift assays demonstrated specific protein binding to this region under oxygen limitation. Similar to the <jats:italic>S. cerevisiae<\/jats:italic> heme sensor system, the promoter was induced by Co<jats:sup>2+<\/jats:sup>. Cyanide was not able to mimic the effect of oxygen limitation. The activation mechanism of <jats:italic>PsADH2<\/jats:italic> also, in this respect, has similarities to the mammalian HIF\u20101 system, which is inducible by Co<jats:sup>2+<\/jats:sup> but not by cyanide. Thus, the very first promoter analysis in <jats:italic>P. stipitis<\/jats:italic> revealed a hitherto unknown mechanism of oxygen sensing in yeast. Copyright \u00a9 2002 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/yea.933","type":"journal-article","created":{"date-parts":[[2002,12,18]],"date-time":"2002-12-18T14:08:26Z","timestamp":1040220506000},"page":"39-51","source":"Crossref","is-referenced-by-count":47,"title":["Analysis of the hypoxia\u2010induced <i>ADH2<\/i> promoter of the respiratory yeast <i>Pichia stipitis<\/i> reveals a new mechanism for sensing of oxygen limitation in yeast"],"prefix":"10.1002","volume":"20","author":[{"given":"Volkmar","family":"Passoth","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marita","family":"Cohn","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bernd","family":"Sch\u00e4fer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B\u00e4rbel","family":"Hahn\u2010H\u00e4gerdal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ulrich","family":"Klinner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2002,11,29]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.1030410103"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.1030430303"},{"key":"e_1_2_6_4_1","volume-title":"Current Protocols in Molecular Biology","author":"Ausubel FM","year":"1991"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2002.02724.x"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/yea.320090710"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00292085"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2000.02188.x"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(76)90527-3"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00251847"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.64.4.1350-1358.1998"},{"key":"e_1_2_6_12_1","doi-asserted-by":"crossref","first-page":"2363","DOI":"10.1128\/AEM.65.6.2363-2368.1999","article-title":"Transcriptional control of ADH genes in the xylose fermenting yeast Pichia stipitis","volume":"65","author":"Cho J\u2010Y","year":"1999","journal-title":"Appl Environ Microbiol"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-143-2-303"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00138012"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s002530100790"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00419911"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1996.tb02631.x"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/ki.1997.67"},{"key":"e_1_2_6_19_1","unstructured":"EliassonA.2000.Ethanol Production by Recombinant and Natural Xylose\u2010utilizing Yeasts. 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