{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,6]],"date-time":"2026-05-06T20:38:17Z","timestamp":1778099897649,"version":"3.51.4"},"reference-count":15,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2003,2,26]],"date-time":"2003-02-26T00:00:00Z","timestamp":1046217600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Yeast"],"published-print":{"date-parts":[[2003,4,15]]},"abstract":"Abstract<\/jats:title>Histoplasma capsulatum<\/jats:italic> possesses a branched mitochondrial electron transport chain, with both cyanide\u2010sensitive and \u2010insensitive oxygen\u2010consuming activities. The latter, carried out by a single subunit enzyme termed \u2018alternative oxidase\u2019, is the focus of this report. AOX1<\/jats:italic> cDNA clones were isolated and direct evidence that the cDNA ORF encodes functional alternative oxidase is reported. Also reported are the generation of an antiserum to the AOX1<\/jats:italic> protein product, and specific detection in vivo<\/jats:italic> of the mRNA and protein products of the AOX1<\/jats:italic> gene. Finally, initial studies of regulation of H. capsulatum AOX1<\/jats:italic> gene expression demonstrated that RNA abundance was increased after hydrogen peroxide\u2010mediated oxidative stress and after inhibition of mitochondrial electron transport enzymes with antimycin A or sodium cyanide. This pattern of regulation is consistent with the hypothesis that alternative oxidase contributes to survival of H. capsulatum<\/jats:italic> after oxidative or metabolic stress and may be important for virulence of this pathogenic organism. The GenBank Accession Nos for the cDNA sequences reported in this paper are AF133236, AF133237 (AOX1<\/jats:italic>). 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