{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,31]],"date-time":"2026-10-31T18:47:44Z","timestamp":1793472464550,"version":"4.5.0"},"reference-count":19,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2007,7,20]],"date-time":"2007-07-20T00:00:00Z","timestamp":1184889600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Zoo Biology"],"published-print":{"date-parts":[[2007,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The design and implementation of assisted reproductive technology to improve genetic diversity and augment captive populations is an important but rarely applied research field in reptiles. Using the corn snake (<jats:italic>Elaphe gutatta<\/jats:italic>) as a model, the Henry Doorly Zoo recently produced offspring born as a result of artificial insemination using both fresh, diluted semen, and diluted semen stored at refrigeration for 3 days. Semen was collected noninvasively from sexually mature male corn snakes using a gentle massaging technique, extended in medium then inseminated into the oviducts of adult females. Using molecular genetic techniques to confirm or refute the success of the insemination using primers developed for the black rat snake, <jats:italic>Elaphe obsolete<\/jats:italic>, all possible parents and offspring genotypes were evaluated. A paternity\u2010by\u2010exclusion analysis verified that the offspring were in fact a result of artificial insemination. Zoo Biol 26:363\u2013369, 2007. \u00a9 2007 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/zoo.20144","type":"journal-article","created":{"date-parts":[[2007,7,20]],"date-time":"2007-07-20T21:16:48Z","timestamp":1184966208000},"page":"363-369","source":"Crossref","is-referenced-by-count":37,"title":["Successful artificial insemination in the corn snake, <i>Elaphe gutatta<\/i>, using fresh and cooled semen"],"prefix":"10.1002","volume":"26","author":[{"given":"Kelli J.","family":"Mattson","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alyse","family":"De Vries","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susie M.","family":"McGuire","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jessi","family":"Krebs","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edward E.","family":"Louis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naida M.","family":"Loskutoff","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2007,7,20]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1095-8312.1993.tb00933.x"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1471-8286.2003.00363.x"},{"key":"e_1_2_1_4_1","first-page":"520","article-title":"Isolation and characterization of microsatellite markers in the broad\u2010headed snake Hoplocephalus bungaroides","volume":"8","author":"Burns EL","year":"1999","journal-title":"Mol Ecol"},{"key":"e_1_2_1_5_1","first-page":"34","article-title":"Male combat in venomous snakes","volume":"4","author":"Eipper SC","year":"2002","journal-title":"J Victorian Assoc Amateur Herpetol"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.hdy.6800210"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/jhered\/93.1.63"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-294X.2005.02438.x"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1037\/0735-7044.101.2.228"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/0016-6480(76)90015-0"},{"key":"e_1_2_1_11_1","first-page":"522","article-title":"Establishing paternity in whooping cranes (Grus americana) by DNA analysis","volume":"109","author":"Longmire JL","year":"1982","journal-title":"Auk"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-294x.1999.00720.x"},{"key":"e_1_2_1_13_1","first-page":"629","article-title":"Mating\u2010induced ovaria recrudescence in the red\u2010sided garter snake","volume":"166","author":"Mendoca MT","year":"1990","journal-title":"J Comp Physiol A"},{"key":"e_1_2_1_14_1","unstructured":"MengdenGA PlatzCG HubbardR QuinnH.1980.Semen collection freezing and artificial insemination in snakes. SSAR Contibutions to Herpetology Number 1 Reproductive Biology and Diseases of Captive Reptiles. p71\u201378."},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-294X.1994.tb00125.x"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-294X.1999.00530.x"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1748-1090.1989.tb03277.x"},{"key":"e_1_2_1_18_1","volume-title":"Reproductive husbandry of pythons and boas","author":"Ross RA","year":"1990"},{"key":"e_1_2_1_19_1","volume-title":"Molecular cloning: a laboratory manual 2nd ed","author":"Sambrook J","year":"1989"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/jez.1154"}],"container-title":["Zoo Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fzoo.20144","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/zoo.20144","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,14]],"date-time":"2023-11-14T20:16:18Z","timestamp":1699992978000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/zoo.20144"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,7,20]]},"references-count":19,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2007,9]]}},"alternative-id":["10.1002\/zoo.20144"],"URL":"https:\/\/doi.org\/10.1002\/zoo.20144","archive":["Portico"],"relation":{},"ISSN":["0733-3188","1098-2361"],"issn-type":[{"value":"0733-3188","type":"print"},{"value":"1098-2361","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,7,20]]}}}