{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,30]],"date-time":"2026-03-30T12:10:57Z","timestamp":1774872657645,"version":"3.50.1"},"reference-count":7,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2008,1,31]],"date-time":"2008-01-31T00:00:00Z","timestamp":1201737600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Zoo Biology"],"published-print":{"date-parts":[[2008,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Semen collection and preservation is the first step toward the development of an artificial insemination program in endangered <jats:italic>Pteropus<\/jats:italic> spp. Semen was collected by manual stimulation from a single \u201chuman\u2010habituated\u201d <jats:italic>P. alecto<\/jats:italic>. Manual stimulation resulted in the successful collection of motile spermatozoa on 17 of 34 attempts. The semen had a pH of 8.2 (<jats:italic>n<\/jats:italic>=2). With the exception of volume, seminal characteristics (concentration, motility, acrosome and plasma membrane status) were similar to those collected previously by electro\u2010ejaculation. Zoo Biol 27:159\u2013164, 2008. \u00a9 2008 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/zoo.20169","type":"journal-article","created":{"date-parts":[[2008,1,31]],"date-time":"2008-01-31T19:52:39Z","timestamp":1201809159000},"page":"159-164","source":"Crossref","is-referenced-by-count":3,"title":["Collection of semen by manual stimulation and ejaculate characteristics of the black flying\u2010fox (<i>Pteropus alecto<\/i>)"],"prefix":"10.1002","volume":"27","author":[{"given":"Debbie F.","family":"Melville","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elizabeth G.","family":"Crichton","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Trish","family":"Paterson\u2010Wimberley","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Steve D.","family":"Johnston","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2008,1,31]]},"reference":[{"key":"e_1_2_1_2_1","volume-title":"Proceedings of bovine short course","author":"Barth AD","year":"1995"},{"key":"e_1_2_1_3_1","first-page":"10","volume-title":"Abnormal morphology of bovine spermatozoa","author":"Barth AD","year":"1989"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2005.02.016"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1530\/reprod\/118.2.273"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-08-057109-6.50011-2"},{"key":"e_1_2_1_7_1","first-page":"747","volume-title":"Marshall's physiology of reproduction vol 2: reproduction in the male","author":"Watson PF","year":"1990"},{"key":"e_1_2_1_8_1","first-page":"429","volume-title":"Wild mammals in captivity: principles and techniques","author":"Wildt DE","year":"1997"}],"container-title":["Zoo Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fzoo.20169","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/zoo.20169","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,14]],"date-time":"2023-11-14T20:22:47Z","timestamp":1699993367000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/zoo.20169"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,1,31]]},"references-count":7,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2008,3]]}},"alternative-id":["10.1002\/zoo.20169"],"URL":"https:\/\/doi.org\/10.1002\/zoo.20169","archive":["Portico"],"relation":{},"ISSN":["0733-3188","1098-2361"],"issn-type":[{"value":"0733-3188","type":"print"},{"value":"1098-2361","type":"electronic"}],"subject":[],"published":{"date-parts":[[2008,1,31]]}}}