{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T03:31:02Z","timestamp":1761708662995},"reference-count":46,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2011,5,2]],"date-time":"2011-05-02T00:00:00Z","timestamp":1304294400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Zoo Biology"],"published-print":{"date-parts":[[2012,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>This work was performed as part of a multi\u2010year study to determine the cause of the low fertility in captive rockhopper penguins (<jats:italic>Eudyptes chrysocome chrysocome<\/jats:italic>) and attempt to increase the fertility through artificial insemination (AI). Semen collection and characterization was performed on 14 male rockhopper penguins. The samples were evaluated for volume, sperm concentration, and sperm quality (motility, forward motility, viability, and morphology). There was a large variation between individuals and between collections for each individual. Mean volume of ejaculate was 0.24\u2009ml. Mean concentration was 47.09 \u00d7 10<jats:sup>6<\/jats:sup>\u2009sperm\/ml. Mean number of sperm per collection was 6.57\u00d7 10<jats:sup>6<\/jats:sup>. The mean motility was 49.4%. Mean forward motility was 1.7. Mean percentage of living sperm was 88.9%. Mean percentage of sperm with normal morphology was 69.4%. AI was performed on a total of 10 females using pooled semen samples. The birds were also allowed to naturally mate. Ten eggs were laid and three fertile eggs were produced, one of them hatched but died within 24\u2009hr. Paternity testing was performed using 12 microsatellite loci, but unfortunately due to insufficient variability, the paternity of the chick and two embryos could not be determined. Zoo Biol 31: 166\u2013180, 2012. \u00a9 2011 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/zoo.20390","type":"journal-article","created":{"date-parts":[[2011,5,2]],"date-time":"2011-05-02T12:02:48Z","timestamp":1304337768000},"page":"166-180","source":"Crossref","is-referenced-by-count":12,"title":["Semen Characteristics and artificial insemination in rockhopper penguins (<i>Eudyptes chrysocome chrysocome<\/i>)"],"prefix":"10.1002","volume":"31","author":[{"given":"Jennifer","family":"Waldoch","sequence":"first","affiliation":[{"name":"Indianapolis Zoo  1200 West Washington Street Indianapolis Indiana"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tammy","family":"Root","sequence":"additional","affiliation":[{"name":"Indianapolis Zoo  1200 West Washington Street Indianapolis Indiana"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jean M.","family":"Dubach","sequence":"additional","affiliation":[{"name":"Department of Conservation Sciences Chicago Zoological Society  Brookfield Illinois"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jeffry","family":"Proudfoot","sequence":"additional","affiliation":[{"name":"Indianapolis Zoo  1200 West Washington Street Indianapolis Indiana"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jan","family":"Ramer","sequence":"additional","affiliation":[{"name":"Indianapolis Zoo  1200 West Washington Street Indianapolis Indiana"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2011,5,2]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.3382\/ps.0620683"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.4067\/S0716-078X2002000400014"},{"key":"e_1_2_7_4_1","unstructured":"BinghamM. 2005. Rockhopper Penguin. International Penguin Conservation Work Group. Downloaded fromhttp:\/\/www.penguins.clon August 26 2005."},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1139\/z76-135"},{"key":"e_1_2_7_6_1","unstructured":"BirdLife International. 2008. Species factsheet: Eudyptes chrysocome. 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