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Pre\u2010release Bd detection can be confounded by imperfect animal sampling and the absence of animals. In Study 1, we used historical Bd\u2010positive sites, to concurrently evaluate water filtrates and mouth bar (tadpoles) or skin swab (caudates) samples for Bd using molecular beacon realtime PCR. In Study 2, during a natural outbreak, we used PCR to detect Bd from zoospore\u2010attracting keratin baits (three avian, three snake species). In Study 1, no captured animals (<jats:italic>n<\/jats:italic>\u2009=\u2009116) exhibited clinical signs, although 10.6% were positive, representing three of seven species sampled. In contrast, 5.4% of water filters (<jats:italic>n<\/jats:italic>\u2009=\u200956) were Bd\u2010positive. In Study 2, after short incubation times, a single duck down feather tested Bd\u2010positive. In conclusion, Bd was detected in asymptomatic amphibians and water filtrate at two sites, and from water only, at two other sites. With continued refinement, semi\u2010quantitative Bd water filtrate screening could better define zoospore\u2010specific disease risk, allowing better characterization of the free\u2010living phase of the organism's life cycle. Finally, these results suggest wild aquatic birds (e.g., waterfowl) should be systematically explored as a means of Bd spread. Since large numbers of aquatic birds migrate, even low Bd transfer rates could be a significant means for disease dissemination. 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