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Therefore, we examined viability of semen samples from captive golden lanceheads diluted in coconut\u2010water based extender over a 48\u2010h period of cooling (5\u00b0C). Semen evaluations were performed immediately after the dilution and at 6, 24, and 48\u2009h, using computer\u2010assisted sperm analysis and stains to assess plasma membrane and acrosomal status. Our findings showed that the extender and protocol employed here were effective in preserving golden lancehead pitviper spermatozoa for a short periods of time (48\u2009h), allowing semen samples to be transported to distant locations for immediate use without the setbacks of cryopreservation.<\/jats:p>","DOI":"10.1002\/zoo.21590","type":"journal-article","created":{"date-parts":[[2021,2,14]],"date-time":"2021-02-14T02:56:05Z","timestamp":1613271365000},"page":"256-260","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Short\u2010term cold storage of seminal samples of the golden lancehead pitviper (<i>Bothrops insularis)<\/i>"],"prefix":"10.1002","volume":"40","author":[{"given":"Kalena Barros","family":"da Silva","sequence":"first","affiliation":[{"name":"Departamento de Cirurgia, Faculdade de Medicina Veterin\u00e1ria e Zootecnia Universidade S\u00e3o Paulo S\u00e3o Paulo S\u00e3o Paulo Brazil"},{"name":"Laborat\u00f3rio de Ecologia e Evolu\u00e7\u00e3o Instituto Butantan S\u00e3o Paulo S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mayra Hespanhol","family":"Frediani","sequence":"additional","affiliation":[{"name":"Departamento de Reprodu\u00e7\u00e3o Animal, Faculdade de Medicina Veterin\u00e1ria e Zootecnia Universidade S\u00e3o Paulo S\u00e3o Paulo S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel de Souza Ramos","family":"Angrimani","sequence":"additional","affiliation":[{"name":"Departamento de Reprodu\u00e7\u00e3o Animal, Faculdade de Medicina Veterin\u00e1ria e Zootecnia Universidade S\u00e3o Paulo S\u00e3o Paulo S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rafaela Zani","family":"Coeti","sequence":"additional","affiliation":[{"name":"Departamento de Cirurgia, Faculdade de Medicina Veterin\u00e1ria e Zootecnia Universidade S\u00e3o Paulo S\u00e3o Paulo S\u00e3o Paulo Brazil"},{"name":"Laborat\u00f3rio de Ecologia e Evolu\u00e7\u00e3o Instituto Butantan S\u00e3o Paulo S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ricardo Jos\u00e9 Garcia","family":"Pereira","sequence":"additional","affiliation":[{"name":"Departamento de Reprodu\u00e7\u00e3o Animal, Faculdade de Medicina Veterin\u00e1ria e Zootecnia Universidade S\u00e3o Paulo S\u00e3o Paulo S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marc\u00edlio","family":"Nichi","sequence":"additional","affiliation":[{"name":"Departamento de Reprodu\u00e7\u00e3o Animal, Faculdade de Medicina Veterin\u00e1ria e Zootecnia Universidade S\u00e3o Paulo S\u00e3o Paulo S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Selma Maria","family":"de Almeida\u2010Santos","sequence":"additional","affiliation":[{"name":"Departamento de Cirurgia, Faculdade de Medicina Veterin\u00e1ria e Zootecnia Universidade S\u00e3o Paulo S\u00e3o Paulo S\u00e3o Paulo Brazil"},{"name":"Laborat\u00f3rio de Ecologia e Evolu\u00e7\u00e3o Instituto Butantan S\u00e3o Paulo S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,2,11]]},"reference":[{"key":"e_1_2_9_2_1","volume-title":"Anais do VIII Congresso Brasileiro de Herpetologia","author":"Almeida\u2010santos S. 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