{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,8,22]],"date-time":"2023-08-22T04:32:30Z","timestamp":1692678750976},"reference-count":32,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2021,6,14]],"date-time":"2021-06-14T00:00:00Z","timestamp":1623628800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Zoo Biology"],"published-print":{"date-parts":[[2021,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Raising insects in a laboratory for release into the wild is a common conservation practice, but maintaining breeding colonies year\u2010round can be limited by seasonal food availability. Food availability is particularly challenging for insects which depend on specific host plants. For example, our early efforts to rear the imperiled Atala hairstreak butterfly (<jats:italic>Eumaeus atala<\/jats:italic> Poey) resulted in colony failure during winter due to lack of food. To overcome this barrier, we developed a modified freeze\u2010dried host plant diet to support the colony. The diet consisted of reconstituted freeze\u2010dried leaves and stems from fresh\u2010growth coontie (<jats:italic>Zamia integrifolia<\/jats:italic>), the host plant for the Atala butterflies. We fed larvae less than 9\u2009mm on this freeze\u2010dried diet and transferred them to live coontie plants after they were more than 9\u2009mm. We reared a colony of Atala butterflies using these methods for 859 days, resulting in more than 3400 animals released into the wild. Comparing colony counts during that time period to the 548 days we reared them without modified freeze\u2010dried diet showed a clear benefit in using freeze\u2010dried diet. A growth trial (<jats:italic>N<\/jats:italic>\u2009=\u200940) of larvae fed on only freeze\u2010dried diet compared to larvae fed on fresh coontie cuttings found no significant difference in larval or pupal development between groups (<jats:italic>p<\/jats:italic> \u2009= \u20090.71 and <jats:italic>p<\/jats:italic> \u2009= \u20090.47, respectively). We, therefore, conclude that the freeze\u2010dried diet provided an appropriate alternative for Atala colonies when fresh growth from the host plant is unavailable, and we recommend use of this technique for raising other host plant\u2010dependent insect species of conservation concern.<\/jats:p>","DOI":"10.1002\/zoo.21628","type":"journal-article","created":{"date-parts":[[2021,6,14]],"date-time":"2021-06-14T12:06:11Z","timestamp":1623672371000},"page":"429-435","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["A modified diet to support conservation of the Atala hairstreak butterfly (<i>Eumaeus atala<\/i> Poey)"],"prefix":"10.1002","volume":"40","author":[{"given":"Elizabeth","family":"Braatz","sequence":"first","affiliation":[{"name":"Disney's Animals, Science, and Environment The Walt Disney Company  Lake Buena Vista Florida USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jamie","family":"Sincage","sequence":"additional","affiliation":[{"name":"Disney's Animals, Science, and Environment The Walt Disney Company  Lake Buena Vista Florida USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zachariah J.","family":"Gezon","sequence":"additional","affiliation":[{"name":"Disney's Animals, Science, and Environment The Walt Disney Company  Lake Buena Vista Florida USA"},{"name":"The Butterfly Biosphere Thanksgiving Point Institute  Lehi Utah USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lily T.","family":"Maynard","sequence":"additional","affiliation":[{"name":"Disney's Animals, Science, and Environment The Walt Disney Company  Lake Buena Vista Florida USA"},{"name":"Global Conservation Cincinnati Zoo &amp; Botanical Garden  Cincinnati Ohio USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amanda","family":"Ardente","sequence":"additional","affiliation":[{"name":"Ardente Veterinary Nutrition, LLC  Alachua Florida USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anne","family":"Savage","sequence":"additional","affiliation":[{"name":"Proyecto Titi, Inc.  Orlando Florida USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kathleen E.","family":"Sullivan","sequence":"additional","affiliation":[{"name":"Disney's Animals, Science, and Environment The Walt Disney Company  Lake Buena Vista Florida USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shannon","family":"Livingston","sequence":"additional","affiliation":[{"name":"Disney's Animals, Science, and Environment The Walt Disney Company  Lake Buena Vista Florida USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eduardo V.","family":"Valdes","sequence":"additional","affiliation":[{"name":"Disney's Animals, Science, and Environment The Walt Disney Company  Lake Buena Vista Florida USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,6,14]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1570-7458.1989.tb01303.x"},{"key":"e_1_2_9_3_1","unstructured":"Ardente A. 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