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While roscovitine and serum starvation have been successfully applied in domestic and wild terrestrial mammals, their use in aquatic mammals remains poorly investigated. The development of cellular reprogramming protocols in Antillean manatees, a flagship and endangered aquatic species, represents a promising tool for advancing conservation efforts. This study evaluated the effects of 10\u2009\u00b5M roscovitine (12 and 24\u2009h) and serum starvation (0.5% serum for 24\u2009h) on G\n                    <jats:sub>0<\/jats:sub>\n                    \/G\n                    <jats:sub>1<\/jats:sub>\n                    phase synchronization and viability in somatic cells of the Antillean manatee using flow cytometry. Additionally, we assessed cell morphology, viability, and apoptosis through microscopy\u2010based analyses. The results showed that neither serum starvation (68.4%) nor roscovitine treatment (12\u2009h: 84.3%; 24\u2009h: 79.3%) enhanced G\n                    <jats:sub>0<\/jats:sub>\n                    \/G\n                    <jats:sub>1<\/jats:sub>\n                    synchronization compared to the control group (83.4%). Serum starvation significantly reduced the proportion of G\n                    <jats:sub>0<\/jats:sub>\n                    \/G\n                    <jats:sub>1<\/jats:sub>\n                    \u2010phase cells compared to the 12\u2009h roscovitine group and the control (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05). No differences were observed in morphology or viability across groups (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&gt;\u20090.05). However, extended exposure to roscovitine (24\u2009h) increased apoptotic markers compared to the control group. In summary, although roscovitine (12\u2009h) and serum starvation did not compromise cell viability, neither method effectively induced G\n                    <jats:sub>0<\/jats:sub>\n                    \/G\n                    <jats:sub>1<\/jats:sub>\n                    synchronization under the conditions tested. Studies are needed to elucidate the\n                    <jats:italic>in vitro<\/jats:italic>\n                    behavior of Antillean manatee somatic cells and to optimize protocols for nuclear reprogramming applications.\n                  <\/jats:p>","DOI":"10.1002\/zoo.70108","type":"journal-article","created":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T12:58:15Z","timestamp":1788267495000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Effects of Roscovitine and Serum Starvation on the Viability and G\n                    <sub>0<\/sub>\n                    \/G\n                    <sub>1<\/sub>\n                    Synchronization in Somatic Cells from the Antillean Manatee (\n                    <i>Trichechus manatus manatus<\/i>\n                    , Linnaeus 1758)"],"prefix":"10.1002","author":[{"given":"Luanna Lorenna Vieira","family":"Rodrigues","sequence":"first","affiliation":[{"name":"Laboratory of Animal Biotechnology Federal Rural University of Semi\u2010Arid Mossor\u00f3 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leonardo Vitorino Costa","family":"de Aquino","sequence":"additional","affiliation":[{"name":"Laboratory of Animal Biotechnology Federal Rural University of Semi\u2010Arid Mossor\u00f3 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lhara Ricarliany Medeiros","family":"de Oliveira","sequence":"additional","affiliation":[{"name":"Laboratory of Animal Biotechnology Federal Rural University of Semi\u2010Arid Mossor\u00f3 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jo\u00e3o Vitor","family":"da Silva Viana","sequence":"additional","affiliation":[{"name":"Laboratory of Animal Biotechnology Federal Rural University of Semi\u2010Arid Mossor\u00f3 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah Sant'Anna","family":"Maranh\u00e3o","sequence":"additional","affiliation":[{"name":"Laboratory of Experimental Oncology Federal University of Cear\u00e1 Fortaleza Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7871-2513","authenticated-orcid":false,"given":"Maria Claudia dos Santos","family":"Luciano","sequence":"additional","affiliation":[{"name":"Ceara Cancer Institute Fortaleza Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0370-4447","authenticated-orcid":false,"given":"Radan Elvis Matias","family":"de Oliveira","sequence":"additional","affiliation":[{"name":"Laboratory of Applied Animal Morphophysiology Federal Rural University of Semi\u2010Arid Mossor\u00f3 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F\u00e1bia Oliveira","family":"Luna","sequence":"additional","affiliation":[{"name":"National Center for Research and Conservation of Aquatic Mammals by the Chico Mendes Institute for Biodiversity Conservation S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2640-6714","authenticated-orcid":false,"given":"Fernanda Loffler Niemeyer","family":"Attademo","sequence":"additional","affiliation":[{"name":"Laboratory of Animal Biotechnology Federal Rural University of Semi\u2010Arid Mossor\u00f3 Brazil"},{"name":"National Center for Research and Conservation of Aquatic Mammals by the Chico Mendes Institute for Biodiversity Conservation S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claudia","family":"Pessoa","sequence":"additional","affiliation":[{"name":"Laboratory of Experimental Oncology Federal University of Cear\u00e1 Fortaleza 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