{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T10:57:40Z","timestamp":1792061860348,"version":"4.4.0"},"reference-count":72,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T00:00:00Z","timestamp":1792022400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T00:00:00Z","timestamp":1792022400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Zoo Biology"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Diet is a crucial factor in modulating the gut microbiome, and understanding these interactions in captive primates is essential for their health and welfare. This study assessed the impact of the transition to a high\u2010fiber, low\u2010sugar diet on the gut microbiome, behavior, and physical health of five captive Bornean orangutans (\n                    <jats:italic>Pongo pygmaeus<\/jats:italic>\n                    ). We hypothesized that altering dietary patterns would significantly influence gut microbiome diversity and composition, as well as behavioral and physiological indicators. Over 12 weeks, the orangutans were gradually transitioned from a fruit\u2010rich diet to a high\u2010fiber, fruitless diet, with progressive dietary changes implemented every 2 weeks. Fecal samples were collected throughout the study, and the microbial diversity and composition were analyzed using 16S rRNA amplicon sequencing. Statistical analyses using linear mixed\u2010effects models evaluated changes in microbial diversity and composition over time. In parallel, fecal consistency, behavior, and body condition scores were monitored regularly. The results showed significant changes in microbial richness and diversity associated with the dietary transition, with specific taxa, such as the genus\n                    <jats:italic>Prevotella<\/jats:italic>\n                    and the family Ruminococcaceae, increasing in abundance. The microbiome shifts were accompanied by changes in body condition and fecal scores, as well as reduced stress\u2010related behaviors (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05), increased feeding duration (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.001), and increased social behaviors, including intraspecific interactions (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05). This study provides preliminary evidence that a high\u2010fiber, low\u2010sugar dietary approach may influence gut microbiome composition and selected behavioral indicators in zoo\u2010housed orangutans.\n                  <\/jats:p>","DOI":"10.1002\/zoo.70122","type":"journal-article","created":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T10:40:49Z","timestamp":1792060849000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Impact of a High\u2010Fiber, Fruitless Diet on the Gut Microbiome, and Health of Captive Orangutans"],"prefix":"10.1002","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-6639-3704","authenticated-orcid":false,"given":"Camille","family":"Duvivier","sequence":"first","affiliation":[{"name":"Cabinet V\u00e9t\u00e9rinaire du Lion Le Pradet France"},{"name":"La M\u00e9nagerie, Le Zoo du Jardin des Plantes, Museum National d'Histoire Naturelle Paris 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le D\u00e9veloppement de l'Elevage (SELMET\u2010LRDE) Corte France"},{"name":"EA 7310, Laboratoire de Virologie Universit\u00e9 de Corse Corte France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6926-2194","authenticated-orcid":false,"given":"Elianne","family":"Piloto\u2010Sardi\u00f1as","sequence":"additional","affiliation":[{"name":"ANSES, INRAE, Ecole Nationale V\u00e9t\u00e9rinaire d'Alfort, UMR BIPAR, Laboratoire de Sant\u00e9 Animale Maisons\u2010Alfort France"},{"name":"Animal Health Division, National Center for Animal and Plant Health San Jos\u00e9 de las Lajas Mayabeque Cuba"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lianet","family":"Abuin\u2010Denis","sequence":"additional","affiliation":[{"name":"ANSES, INRAE, Ecole Nationale V\u00e9t\u00e9rinaire d'Alfort, UMR BIPAR, Laboratoire de Sant\u00e9 Animale Maisons\u2010Alfort France"},{"name":"Animal Biotechnology Department Center for Genetic Engineering and Biotechnology Havana Cuba"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3805-8374","authenticated-orcid":false,"given":"Aude","family":"Bourgeois","sequence":"additional","affiliation":[{"name":"La M\u00e9nagerie, Le Zoo du Jardin des Plantes, Museum National d'Histoire Naturelle Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-1447-0795","authenticated-orcid":false,"given":"C\u00e9cile","family":"Maillochaud","sequence":"additional","affiliation":[{"name":"ENVA, Ecole Nationale V\u00e9t\u00e9rinaire d'Alfort Maisons\u2010Alfort France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana\u00efs","family":"Robin","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Sorbonne Paris Nord Villetaneuse 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