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We include a classical isolation method for soluble tubulin heterodimer as the first basic purification protocol. In addition, we show how to analyze the tubulin and MAPs obtained after a phosphocellulose chromatography purification procedure. This unit also details a powerful and simple method to determine the native state of the purified tubulin based on one\u2010dimensional electrophoresis under nondenaturing conditions (<jats:sc><jats:italic>UNIT 6.5<\/jats:italic><\/jats:sc>). The last protocol describes the application of a new technique that allows visualizing the quality of polymerized microtubules based on atomic force microscopy (AFM). <jats:italic>Curr. Protoc. Cell Biol<\/jats:italic>. 39:3.29.1\u20103.29.28. \u00a9 2008 by John Wiley &amp; Sons, Inc.<\/jats:p>","DOI":"10.1002\/0471143030.cb0329s39","type":"journal-article","created":{"date-parts":[[2008,6,30]],"date-time":"2008-06-30T11:23:56Z","timestamp":1214825036000},"source":"Crossref","is-referenced-by-count":14,"title":["Isolation of Microtubules and Microtubule Proteins"],"prefix":"10.1002","volume":"39","author":[{"given":"J.","family":"Avila","sequence":"first","affiliation":[{"name":"Centro de Biolog\u00eda Molecular (CSIC). 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