{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,28]],"date-time":"2026-03-28T23:57:22Z","timestamp":1774742242959,"version":"3.50.1"},"reference-count":31,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2011,11,1]],"date-time":"2011-11-01T00:00:00Z","timestamp":1320105600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["CP Microbiology"],"published-print":{"date-parts":[[2011,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Quorum sensing is a cell\u2010cell signaling process that many bacteria use to regulate gene expression as a function of the density of the population. This phenomenon involves the production, release, and response to small chemical molecules termed autoinducers. Most autoinducers are species\u2010specific; however, one autoinducer called autoinducer\u20102 (AI\u20102) is produced and detected by many species of bacteria and thus can foster inter\u2010species communication. This unit describes two assays to detect and quantify AI\u20102 from biological samples. The first uses a bacterial reporter strain, which produces bioluminescence in response to AI\u20102. The second is an in vitro assay based on a modified version of an AI\u20102 receptor fused to a cyan fluorescent protein and a yellow fluorescent protein. Binding of AI\u20102 to this fusion protein induces a dose\u2010dependent decrease in fluorescence resonance energy transfer (FRET), enabling quantification of the AI\u20102 concentration in the samples. <jats:italic>Curr. Protoc. Microbiol<\/jats:italic>. 23:1C.1.1\u20101C.1.15. \u00a9 2011 by John Wiley &amp; Sons, Inc.<\/jats:p>","DOI":"10.1002\/9780471729259.mc01c01s23","type":"journal-article","created":{"date-parts":[[2011,11,1]],"date-time":"2011-11-01T12:28:47Z","timestamp":1320150527000},"source":"Crossref","is-referenced-by-count":55,"title":["Methods for Analysis of Bacterial Autoinducer\u20102 Production"],"prefix":"10.1002","volume":"23","author":[{"given":"Michiko E.","family":"Taga","sequence":"first","affiliation":[{"name":"Department of Plant and Microbial Biology, University of California  Berkeley California"}]},{"given":"Karina B.","family":"Xavier","sequence":"additional","affiliation":[{"name":"Instituto Gulbenkian de Ciencia, Bacterial Signaling Laboratory  Oeiras Portugal"}]}],"member":"311","published-online":{"date-parts":[[2011,11]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bmc.2010.12.036"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1993.tb01737.x"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1994.tb00422.x"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1128\/jb.179.12.4043-4045.1997"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/415545a"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.C0117-0"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI20195"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.12.7625-7634.2001"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro1916"},{"key":"e_1_2_7_11_1","doi-asserted-by":"crossref","unstructured":"Lowery C.A. 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