{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,7]],"date-time":"2026-02-07T02:26:54Z","timestamp":1770431214906,"version":"3.49.0"},"reference-count":35,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2005,2,3]],"date-time":"2005-02-03T00:00:00Z","timestamp":1107388800000},"content-version":"vor","delay-in-days":13304,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Am. J. Anat."],"published-print":{"date-parts":[[1968,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The maturation of brown adipose cells, derived at various time intervals from interscapular brown fat pads of neonatal rats, has been investigated electron microscopically. The major changes in <jats:italic>in vivo<\/jats:italic> brown adipose cells are related to lipid accumulation. In older animals, fat droplets are separated by a membrane complex, and membranous fragments are seen within large lipid inclusions. The ultrastructural characteristics of monolayer cultures of brown fat cells, isolated from neonatal brown fat pads by tryptic dissociation, and maintained for one to six days on a reconstituted collagen matrix, have also been investigated. Adipose cells <jats:italic>in vitro<\/jats:italic> are morphologically similar to control brown fat cells, and lipid accumulation closely parallels that seen <jats:italic>in vivo. In vitro<\/jats:italic> lipid droplet coalescence, impossible to visualize <jats:italic>in vivo<\/jats:italic>, has been documented by time\u2010lapse cinematography, and membrane complexes similar to those observed <jats:italic>in vivo<\/jats:italic> are seen. Major changes in cultivated fat cells, compared to <jats:italic>in vivo<\/jats:italic> cells, involve extensive glycogen deposition and breakdown of cytoplasmic ribosomal rosettes. Older cultures contain adipose cells exhibiting characteristics of white fat cells. Effects of tissue culture environment upon the brown fat cells, and the possible conversion of the brown fat cell into a white fat cell are discussed.<\/jats:p>","DOI":"10.1002\/aja.1001230203","type":"journal-article","created":{"date-parts":[[2005,2,26]],"date-time":"2005-02-26T15:01:26Z","timestamp":1109430086000},"page":"255-281","source":"Crossref","is-referenced-by-count":22,"title":["Morphological features of brown adipose cell maturation <i>in vivo<\/i> and <i>in vitro<\/i>"],"prefix":"10.1002","volume":"123","author":[{"given":"Robert F.","family":"Dyer","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,2,3]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02978388"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.8.1.83"},{"key":"e_1_2_1_4_1","first-page":"118","volume-title":"Manometric Techniques and Tissue Metabolism","author":"Cohen P. 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