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In late 2\u2010cell embryos, some of these nucleoli acquire a peripheral zone of granules, while others reticulate, forming nucleoli composed of fibrillo\u2010granular cortices and fibrillar cores. The nucleoli of early 4\u2010cell embryos are composed only of fibrils. In the middle of the 4\u2010cell stage, some of the nucleoli acquire a peripheral granular zone, while others reticulate. The reticulated nucleoli of both the late 2\u2010cell and 4\u2010cell embryos can be considered, on the basis of their fine structure, to be definitive nucleoli. Early 8\u2010cell and morula embryos usually contain only two definitive nucleoli per nucleus. <jats:sup>3<\/jats:sup>H\u20105\u2010uridine\u2010pulsed embryos contain label localized in the nucleus, particularly over definitive nucleoli. Nucleolar labeling increases at each successive developmental stage. Beginning at the 8\u2010cell stage, re\u2010incubation in nonradioactive medium results in a significant decrease in nucleolar labeling and an increase in cytoplasmic labeling suggesting that more ribosomal RNA is transferred from the nucleus to the cytoplasm at the later cleavage stages.<\/jats:p>","DOI":"10.1002\/aja.1001260203","type":"journal-article","created":{"date-parts":[[2005,2,26]],"date-time":"2005-02-26T14:41:08Z","timestamp":1109428868000},"page":"151-173","source":"Crossref","is-referenced-by-count":120,"title":["Ultrastructural and autoradiographic studies of mouse cleavage stages"],"prefix":"10.1002","volume":"126","author":[{"given":"Nina","family":"Hillman","sequence":"first","affiliation":[]},{"given":"Richard J.","family":"Tasca","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2005,2,3]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.53.4.730"},{"key":"e_1_2_1_3_1","first-page":"81","volume-title":"The Nucleus","author":"Bernhard W.","year":"1968"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4827(63)90093-4"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/jez.1401580106"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4827(67)90230-3"},{"key":"e_1_2_1_7_1","first-page":"219","volume-title":"Developmental and Metabolic Control Mechanisms and Neoplasia","author":"Brown D. 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