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In electron micrographs exhibiting a large ECS the tissue elements had a uniform electron density. The EMs with little space featured in some instances a moderate swelling of presynaptic terminals and other tissue elements. Brains kept in a cooled medium or a salt solution with MgCl<jats:sub>2<\/jats:sub> added exhibited invariably an abundant ECS. Treating the brain with a 100 mM KCl solution 5\u201390 sec before freezing yielded EMs with a contracted ECS and swollen tissue elements, many of which could be identified as dendritic spines. This effect of KCl was in many experiments prevented by bathing the brain in a salt solution containing 10 mM MgCl<jats:sub>2<\/jats:sub> or in a Ca\u2010free solution.<\/jats:p>","DOI":"10.1002\/ar.1091780306","type":"journal-article","created":{"date-parts":[[2005,2,26]],"date-time":"2005-02-26T08:07:39Z","timestamp":1109405259000},"page":"587-597","source":"Crossref","is-referenced-by-count":4,"title":["Conditions affecting the extracellular space in the frog's forebrain"],"prefix":"10.1002","volume":"178","author":[{"given":"A.","family":"Van Harreveld","sequence":"first","affiliation":[]},{"given":"Janett","family":"Trubatch","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2005,1,27]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1152\/jn.1956.19.3.201"},{"key":"e_1_2_1_3_1","doi-asserted-by":"crossref","first-page":"643","DOI":"10.1152\/ajplegacy.1968.214.3.643","article-title":"Extracellular fluid, ionic distribution and exchange in isolated frog brain","volume":"214","author":"Bradbury M. 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