{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,26]],"date-time":"2025-10-26T14:16:07Z","timestamp":1761488167990},"reference-count":46,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2008,12,16]],"date-time":"2008-12-16T00:00:00Z","timestamp":1229385600000},"content-version":"vor","delay-in-days":4002,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["BioFactors"],"published-print":{"date-parts":[[1998,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The involvement of epidermal growth factor (EGF) and homologous transforming growth factor (TGF) in human kidney development was studied by analyzing their effects on the regulation of DNA synthesis in organ culture and by localizing their cognate receptors. Both peptides significantly increased <jats:sup>3<\/jats:sup>H\u2010thymidine incorporation when added at 10\u2013100 ng\/ml, but not at 1\u20135 ng\/ml. Furthermore, addition of an anti\u2010EGF receptor antibody not only reduced the effect of exogenous EGF (100 ng\/ml) on DNA synthesis but decreased basal <jats:sup>3<\/jats:sup>H\u2010thymidine incorporation. These results indicate that EGF\/TGF\u03b1 are both mitogenic in vitro and further suggest that human fetal kidneys release an endogenous EGF\u2010related substance masking the effects of low amounts of growth factors added to culture medium. Radioautographic analyses show that EGF (100 ng\/ml) increased DNA synthesis in poorly\u2010differentiated cells of the nephrogenic zone, particularly in subcapsular mesenchyme and peritubular cells; although less responsive, epithelial cells in early nephric tubules represented another target of EGF action. The pattern of EGF\/TGF\u03b1 receptor expression was revealed immunohistochemically at different gestational ages and was shown to be related to the proliferation status. It was maximal in condensing nephrogenic cells, relatively high in newly\u2010induced epithelium and cortical branches of ureteric epithelium, low in differentiating nephronic cells and nearly absent from renal stroma and medullary collecting ducts. Together, our results indicate that the EGF\/TGF\u03b1 system is directly involved in the regulation of nephrogenic cell proliferation during human metanephrogenesis and it is progressively down\u2010regulated after conversion of mesenchyme into epithelium.<\/jats:p>","DOI":"10.1002\/biof.5520070404","type":"journal-article","created":{"date-parts":[[2008,12,19]],"date-time":"2008-12-19T00:21:19Z","timestamp":1229646079000},"page":"323-335","source":"Crossref","is-referenced-by-count":9,"title":["Mitogenic effects of EGF\/TGF\u03b1 and immunolocalization of cognate receptors in human fetal kidneys"],"prefix":"10.1002","volume":"7","author":[{"given":"Pierre","family":"Chailler","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Normand","family":"Bri\u00e1re","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2008,12,16]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1159\/000185886"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00862522"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.92.10.4412"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0177(199607)206:3<231::AID-AJA1>3.0.CO;2-J"},{"key":"e_1_2_1_6_2","first-page":"313","article-title":"Epidermal growth factor (EGF) influences DNA synthesis in human fetal kidneys in serum\u2010free organ culture","volume":"1","author":"Bertrand L.","year":"1988","journal-title":"BioFactors"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00309758"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1139\/y88-089"},{"key":"e_1_2_1_9_2","first-page":"113","article-title":"Synergistic influence of epidermal growth factor, insulin and transferrin on human fetal kidney in culture","volume":"3","author":"Bri\u00e9re N.","year":"1991","journal-title":"BioFactors"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02618145"},{"key":"e_1_2_1_11_2","first-page":"F271","article-title":"Regulation of mitogenesis, motogenesis, and tubulogenesis by hepatocyte growth factor in renal collecting duct cells","volume":"36","author":"Cantley L.G.","year":"1994","journal-title":"Am. 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