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The relationship between [\u03b7] and <jats:italic>S<\/jats:italic><jats:inline-graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"graphic\/tex2gif-stack-1.gif\" xlink:title=\"urn:x-wiley:00063525:media:BIP360070511:tex2gif-stack-1\" \/><jats:sub>,w<\/jats:sub> is well represented by the Mandelkern\u2010Flory equation over the entire range of conditions between 0.0013 and 1<jats:italic>M<\/jats:italic> Na<jats:sup>+<\/jats:sup>. An apparent discrepancy between the two methods at moderate to high ionic strengths is probably due to a change in <jats:styled-content><jats:italic>V<\/jats:italic><\/jats:styled-content> with ionic strength. It appears that [\u03b7] is a more sensitive and reliable measure of molecular expansion for native DNA, <jats:italic>S<\/jats:italic><jats:inline-graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"graphic\/tex2gif-stack-2.gif\" xlink:title=\"urn:x-wiley:00063525:media:BIP360070511:tex2gif-stack-2\" \/><jats:sub>,w<\/jats:sub> but is a better index of conformational change in single strands, since [\u03b7] becomes too small to measure conveniently at high ionic strengths. At moderate to high ionic strengths, denaturation leads to a decrease in [\u03b7], although unfolded single strands retain considerable viscosity. At sufficiently low ionic strength, the intrinsic viscosity of the single strands becomes higher than that of native DNA, and the effective volume of a single strand approaches that of the native molecule.<\/jats:p>","DOI":"10.1002\/bip.1969.360070511","type":"journal-article","created":{"date-parts":[[2005,5,27]],"date-time":"2005-05-27T15:16:42Z","timestamp":1117207002000},"page":"765-774","source":"Crossref","is-referenced-by-count":79,"title":["Intrinsic viscosity of native and single\u2010stranded T7 DNA and its relationship to sedimentation coefficient"],"prefix":"10.1002","volume":"7","author":[{"given":"Alan H.","family":"Rosenberg","sequence":"first","affiliation":[]},{"given":"F. 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