{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,3]],"date-time":"2026-04-03T15:27:38Z","timestamp":1775230058337,"version":"3.50.1"},"reference-count":27,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2004,2,1]],"date-time":"2004-02-01T00:00:00Z","timestamp":1075593600000},"content-version":"vor","delay-in-days":9558,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biopolymers"],"published-print":{"date-parts":[[1977,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The heat capacities of the single\u2010stranded and double\u2010stranded forms of polyadenylic acid, polyuridylic acid, and poly(uridylic and adenylic acid) were determined with a drop heat capacity calorimeter. In addition, the temperature dependence of the apparent partial heat capacity (\u03d5<jats:italic>Cp<\/jats:italic>) was measured with a newly developed differential scanning calorimeter. The calculated \u0394<jats:italic>Cp<\/jats:italic> at 28\u00b0C for the transition poly(A)\u00b7poly(A) \u21c4 2 poly(A) was found to be 165 \u00b1 24 cal\/Kmol\u2010base pair, compared with a value of 140 \u00b1 28 for the transition poly(A)\u00b7poly(U) \u21c4 poly(A) + poly(U). The temperature dependence of \u03d5<jats:italic>Cp<\/jats:italic> of single\u2010stranded poly(U) was consistent with the conclusion that it is totally unstacked at temperatures above 15\u00b0C. The temperature dependence of \u03d5<jats:italic>Cp<\/jats:italic> of single\u2010stranded poly(A) was used to determine the base\u2010stacking parameters for poly(A). The experimental results are consistent with a stacking enthalpy change of \u22128.5 \u00b1 0.1 kcal\/mol bases and a cooperativity parameter \u03c3 of 0.57 \u00b1 0.03 for the stacking of adenine bases. These results demonstrate that the heat capacity of single\u2010stranded polynucleotides is greater than that of the double\u2010stranded forms. This increased heat capacity is mainly the result of the temperature dependence of the base\u2010stacking interactions in the single\u2010stranded form.<\/jats:p>","DOI":"10.1002\/bip.1977.360161206","type":"journal-article","created":{"date-parts":[[2004,12,30]],"date-time":"2004-12-30T01:04:22Z","timestamp":1104368662000},"page":"2641-2652","source":"Crossref","is-referenced-by-count":35,"title":["Calorimetric determination of the heat capacity changes associated with the conformational transitions of polyriboadenylic acid and polyribouridylic acid"],"prefix":"10.1002","volume":"16","author":[{"given":"J.","family":"Suurkuusk","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Alvarez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E.","family":"Freire","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.","family":"Biltonen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,2]]},"reference":[{"key":"e_1_2_1_2_2","series-title":"Phys. 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