{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,24]],"date-time":"2026-04-24T03:49:00Z","timestamp":1777002540598,"version":"3.51.4"},"reference-count":39,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2005,2,4]],"date-time":"2005-02-04T00:00:00Z","timestamp":1107475200000},"content-version":"vor","delay-in-days":4417,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cell Motil. Cytoskeleton"],"published-print":{"date-parts":[[1993,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>When present at low concentrations, the fluorescent lipophilic dye, DiOC<jats:sub>6<\/jats:sub>, stains mitochondria in living yeast cells [Pringle et al.: Methods in Cell Biol. 31:357\u2013435, 1989; Weisman et al.: Proc. Natl. Acad. Sci. U.S.A. 87:1076\u20131080, 1990]. However, we found that the nuclear envelope and endoplasmic reticulum were specifically stained if the dye concentration was increased or if certain respiratory\u2010deficient yeast strains were examined. The quality of nuclear envelope staining with DiOC<jats:sub>6<\/jats:sub> was sufficiently sensitive to reveal alterations in the nuclear envelope known as karmellae. These membranes were previously apparent only by electron microscopy. At the high dye concentrations required to stain the nuclear envelope, wild\u2010type cells could no longer grow on non\u2010fermentable carbon sources. In spite of this effect on mitochondrial function, the presence of high dye concentration did not adversely affect cell viability or general growth characteristics when strains were grown under standard conditions on glucose. Consequently, time\u2010lapse confocal microscopy was used to examine organelle dynamics in living yeast cells stained with DiOC<jats:sub>6<\/jats:sub>. These in vivo observations correlated very well with previous electron microscopic studies, including analyses of mitochondria, karmellae, and mitosis. For example, cycles of mitochondrial fusion and division, as well as the changes in nuclear shape and position that occur during mitosis, were readily imaged in time\u2010lapse studies of living DiOC<jats:sub>6<\/jats:sub>\u2010stained cells. This technique also revealed new aspects of nuclear disposition and interactions with other organelles. For example, the nucleus and vacuole appeared to form a structurally coupled unit that could undergo coordinated movements. Furthermore, unlike the general view that nuclear movements occur only in association with division, the nucleus\/vacuole underwent dramatic migrations around the cell periphery as cells exited from stationary phase. In addition to the large migrations or rotations of the nucleus\/vacuole, DiOC<jats:sub>6<\/jats:sub> staining also revealed more subtle dynamics, including the forces of the spindle on the nuclear envelope during mitosis. This technique should have broad application in analyses of yeast cell structure and function. \u00a9 1993 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/cm.970250202","type":"journal-article","created":{"date-parts":[[2005,2,24]],"date-time":"2005-02-24T02:23:33Z","timestamp":1109211813000},"page":"111-128","source":"Crossref","is-referenced-by-count":145,"title":["DiOC<sub>6<\/sub> staining reveals organelle structure and dynamics in living yeast cells"],"prefix":"10.1002","volume":"25","author":[{"given":"Ann J.","family":"Koning","sequence":"first","affiliation":[]},{"given":"Pek Yee","family":"Lum","sequence":"additional","affiliation":[]},{"given":"Jennifer M.","family":"Williams","sequence":"additional","affiliation":[]},{"given":"Robin","family":"Wright","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2005,2,4]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.98.3.934"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/cm.970100120"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.107.4.1369"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1768-322X.1985.tb00388.x"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.8.9.3797"},{"key":"e_1_2_1_7_1","first-page":"59","volume-title":"The Molecular Biology of the Yeast Saccharomyces: Life Cycle and Inheritance","author":"Byers B.","year":"1981"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1101\/SQB.1974.038.01.016"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1128\/jb.124.1.511-523.1975"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(91)90015-Q"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(91)90001-F"},{"key":"e_1_2_1_12_1","first-page":"505","volume-title":"The Molecular Biology of the Yeast Saccharomyces: Life Cycle and Inheritance","author":"Dujon B.","year":"1981"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.104.1.87"},{"key":"e_1_2_1_14_1","first-page":"796","article-title":"Localized secretion of acid phosphatase reflects the pattern of cell\u2010surface growth in Saccharomyces cerevisiae","volume":"254","author":"Field C.","year":"1980","journal-title":"J. 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