{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,3]],"date-time":"2025-11-03T08:58:06Z","timestamp":1762160286025},"reference-count":67,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2004,10,9]],"date-time":"2004-10-09T00:00:00Z","timestamp":1097280000000},"content-version":"vor","delay-in-days":7853,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Comparative Neurology"],"published-print":{"date-parts":[[1983,4,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The axons of the dentate granule cells, the hippocampal mossy fibers, sprout \u201cbackward\u201d into the dentate molecular layer when this is heavily de\u2010nervated. Using the combined Golgi\u2010electron microscopy (EM) technique we now demonstrate that these aberrant supragranular mossy fibers at least in part terminate on granule cell dendrites.<\/jats:p><jats:p>Sprouting of mossy fibers into the dentate molecular layer was induced in adult rats by simultaneous surgical removal of the commissural and ento\u2010rhinal afferents to the fascia dentata. After at least 7 weeks survival, the presence of mossy fiber terminals in the inner part of the dentate molecular layer was demonstrated by light microscopy of Timm\u2010stained cryostat sections and by electron microscopy. In the electron microscope the mossy fiber terminals were identified by their unique structural characteristics, namely, the unusually large size of the terminals, the dense packing of clear synaptic vesicles with a few dense core vesicles intermingled, the presence of asymmetric synaptic contacts with spines and desmosome\u2010like contacts with dendritic shafts, and the continuity with a thin unmyelinated preterminal axon.<\/jats:p><jats:p>Golgi\u2010stained granule cells were first identified in the light microscope, and then, after deimpregnation, the same cells were examined in the electron microscope. In ultrathin, serial sections lesion\u2010induced mossy fiber terminals were found in synaptic contact with spines on proximal dendritic segments of such identified Golgi\u2010impregnated granule cells. From this we conclude that the aberrant, supragranular mossy fibers can innervate dendrites of the parent cell group, the dentate granule cells. The results, moreover, provide an example of reactive synaptogenesis where both the sprouted afferents and its postsynaptic element have been identified.<\/jats:p>","DOI":"10.1002\/cne.902150306","type":"journal-article","created":{"date-parts":[[2004,12,28]],"date-time":"2004-12-28T20:07:17Z","timestamp":1104264437000},"page":"299-311","source":"Crossref","is-referenced-by-count":160,"title":["Lesion\u2010induced mossy fibers to the molecular layer of the rat fascia dentata: Identification of postsynaptic granule cells by the Golgi\u2010EM technique"],"prefix":"10.1002","volume":"215","author":[{"given":"Michael","family":"Frotscher","sequence":"first","affiliation":[]},{"given":"Jens","family":"Zimmer","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2004,10,9]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"crossref","first-page":"851","DOI":"10.1002\/cne.901820508","article-title":"A Golgi study of cell types in the hilar region of the hippocampus in the rat","volume":"782","author":"Amaral D. 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