{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,9]],"date-time":"2025-11-09T07:41:16Z","timestamp":1762674076181},"reference-count":0,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2017,2,1]],"date-time":"2017-02-01T00:00:00Z","timestamp":1485907200000},"content-version":"vor","delay-in-days":15341,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clin Pharma and Therapeutics"],"published-print":{"date-parts":[[1975,2]]},"abstract":"<jats:p>The extent and nature of furosemide (F) binding to human albumin (HA) and to the plasma of 6 children with nephrotic syndrome were studied by equilibrium dialysis at 37\u00b0 C and pH 7.4 with <jats:sup>14<\/jats:sup>C\u2010F. At a total concentration of 3.4 \u00b5\/ml (therapeutic range), the unbound fraction of F to 4 gm per 100 ml HA was 2.79 \u00b1 0.35. The degree of binding was relatively constant from 1.8 to 36 \u00b5\/ml of F concentration. The percentage of unbound F doubled when total concentration of the drug was increased more than 130 times 0.8 to 245 \u00b5\/ml). F has two classes of binding sites (n<jats:sub>1<\/jats:sub> = 1.42, k<jats:sub>1<\/jats:sub> = 5.07 \u00d7 10<jats:sup>4<\/jats:sup> M<jats:sup>\u22121<\/jats:sup>; n<jats:sub>2<\/jats:sub> = 3.4. k<jats:sub>2<\/jats:sub> = 1.58 \u00d7 10<jats:sup>4<\/jats:sup> M<jats:sup>\u22121<\/jats:sup>); interaction with HA involves hydrophobic. ionic. and hydrogen forces. Acetylsalicylic acid (ASA). acetazolamide, diazoxide, phenylbutazone, sulfisoxazole (S), and tolbutamide (T) decreased F binding. Combinations of ASA, S, and T exerted a strong additive displacing effect. The binding of the F metabolite (4\u2010chloro\u20105\u2010sulfamoylanthranilic acid, CSA) was studied at 1.3 and 2.6 \u00b5\/ml. The unbound fraction was 5 times that of F. CSA did not influence F binding. Studies with plasma of 7 healthy adults showed that albumin is the only plasma protein responsible for F binding. The plasma albumin concentration range of the children with nephrotic syndrome was 0.6 to 2.1 gm per 100 ml. There was some correlation between albumin concentration and binding of F (2.8 to 9.6% unbound); this corresponded with findings with HA. Albumin concentrations lower than 2 gm per 100 ml seemed to influence the extent of the unbound fraction of F considerably.<\/jats:p>","DOI":"10.1002\/cpt1975172159","type":"journal-article","created":{"date-parts":[[2016,1,5]],"date-time":"2016-01-05T15:56:27Z","timestamp":1452009387000},"page":"159-166","source":"Crossref","is-referenced-by-count":81,"title":["Furosemide binding to human albumin and plasma of nephrotic children"],"prefix":"10.1111","volume":"17","author":[{"given":"Jozef","family":"Prandota","sequence":"first","affiliation":[]},{"given":"Albert W.","family":"Pruitt","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2017,2]]},"container-title":["Clinical Pharmacology &amp; Therapeutics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fcpt1975172159","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fcpt1975172159","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/ascpt.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cpt1975172159","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,7]],"date-time":"2023-11-07T13:31:46Z","timestamp":1699363906000},"score":1,"resource":{"primary":{"URL":"https:\/\/ascpt.onlinelibrary.wiley.com\/doi\/10.1002\/cpt1975172159"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1975,2]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1975,2]]}},"alternative-id":["10.1002\/cpt1975172159"],"URL":"https:\/\/doi.org\/10.1002\/cpt1975172159","archive":["Portico"],"relation":{},"ISSN":["0009-9236","1532-6535"],"issn-type":[{"value":"0009-9236","type":"print"},{"value":"1532-6535","type":"electronic"}],"subject":[],"published":{"date-parts":[[1975,2]]}}}